Solcà F F, Salomon Y, Eberle A N
Department of Research, University Hospital, Basel, Switzerland.
J Recept Res. 1991;11(1-4):379-90. doi: 10.3109/10799899109066416.
The heterogeneity of melanotropin receptors on B16 sublines was tested by using photoaffinity crosslinking techniques and the superpotent alpha-MSH derivative [Nle4, D-Phe7, 1'-(2-nitro-4-azido-phenylsulfenyl)-Trp9]-alpha- MSH (NAPS-MSH). Specific crosslinking of this compound to B16-F1, B16-F10, B16-M2R or B16-W4 cells revealed three different subtypes of MSH receptor based on SDS-PAGE analysis. Binding of monoiodinated alpha-MSH to these different subclones is saturable and characteristic for a single class of complexes (0.9 nM less than KD less than 1.6 nM). In this article the nature of the different MSH receptor subtypes as well as their possible correlation to the melanogenic potential of a particular cell line is discussed.
利用光亲和交联技术和超强效α-促黑素（α-MSH）衍生物[Nle4，D-Phe7，1'-（2-硝基-4-叠氮基-苯基亚磺酰基）-Trp9]-α-MSH（NAPS-MSH），对B16亚系上促黑素受体的异质性进行了检测。基于SDS-PAGE分析，该化合物与B16-F1、B16-F10、B16-M2R或B16-W4细胞的特异性交联揭示了促黑素受体的三种不同亚型。单碘化α-MSH与这些不同亚克隆的结合具有饱和性，且是一类复合物的特征（0.9 nM＜KD＜1.6 nM）。本文讨论了不同促黑素受体亚型的性质及其与特定细胞系黑素生成潜力的可能关联。
