Walsh Alexandra M, Mustafi Devkumar, Makinen Marvin W, Lee Raphael C
Department of Surgery, University of Chicago Hospitals, MC 6035, 5841 S. Maryland Ave., Chicago, IL 60637, USA.
Ann N Y Acad Sci. 2005 Dec;1066:321-7. doi: 10.1196/annals.1363.029.
The triblock copolymer poloxamer 188 is a non-cytotoxic, nonionic surfactant with both hydrophobic and hydrophilic domains. We show that P188 is able to facilitate the recovery of catalytic activity of heat-denatured lysozyme in dilute solution at low molar ratios of P188:enzyme. Heat-denatured enzyme retained 55% of native activity. After treatment with P188, the enzyme's activity was 85% of native. Because of the low molar ratios used and the non-cytotoxic nature of the compound, P188 may be of potential use in burn therapy.
三嵌段共聚物泊洛沙姆188是一种具有疏水和亲水结构域的无细胞毒性的非离子表面活性剂。我们发现，在P188与酶的低摩尔比下，P188能够促进稀溶液中热变性溶菌酶催化活性的恢复。热变性酶保留了55%的天然活性。用P188处理后，该酶的活性为天然活性的85%。由于使用的摩尔比低以及该化合物无细胞毒性的性质，P188可能在烧伤治疗中具有潜在用途。
