Lazarus Michael
Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA.
Mol Nutr Food Res. 2006 Apr;50(4-5):451-5. doi: 10.1002/mnfr.200500207.
The innate immune system of mammals is able to detect bacteria when they infect local tissue or enter the blood stream, and initiate an immediate immune response. Prostaglandin (PG) E2 is considered as the most important link between the peripheral immune system and the brain. Due to four PGE2 receptors (EP receptors) and their differential expression in various areas of the hypothalamus and brain stem, PGE2 mediates different components of the acute phase reaction. A fever model is discussed in which the preoptic area contains the mechanisms for both hyperthermic and hypothermic responses and EP receptors in the median preoptic area (MnPO) modulate the thermogenic system. The neuron-specific modulation of EP receptors in the MnPO can be critically tested by using Cre-recombinase-mediated DNA recombination in genetically engineered mice. A concept for mice with conditional expression of EP3R and EP4R to investigate the different roles of those receptors in lipopolysaccharide (LPS)-induced fever is presented.
当细菌感染局部组织或进入血流时，哺乳动物的先天免疫系统能够检测到它们，并启动即时免疫反应。前列腺素（PG）E2被认为是外周免疫系统与大脑之间最重要的联系。由于四种PGE2受体（EP受体）及其在下丘脑和脑干不同区域的差异表达，PGE2介导急性期反应的不同组成部分。本文讨论了一种发热模型，其中视前区包含体温升高和降低反应的机制，视前正中区（MnPO）中的EP受体调节产热系统。通过在基因工程小鼠中使用Cre重组酶介导的DNA重组，可以严格测试MnPO中EP受体的神经元特异性调节。本文提出了一种条件性表达EP3R和EP4R的小鼠概念，以研究这些受体在脂多糖（LPS）诱导的发热中的不同作用。
