脉冲场凝胶电泳、核糖体分型和质粒谱分析在鳗弧菌 O1 血清型分型中的比较。
Comparison of Pulsed-Field Gel Electrophoresis, Ribotyping, and Plasmid Profiling for Typing of Vibrio anguillarum Serovar O1.
出版信息
Appl Environ Microbiol. 1995 Apr;61(4):1540-5. doi: 10.1128/aem.61.4.1540-1545.1995.
Abstract
A total of 75 Vibrio anguillarum serogroup O1 strains were studied with respect to their plasmid contents, ribotypes, and pulsed-field gel electrophoresis (PFGE) patterns. Eight plasmid profiles and six ribotypes were demonstrated, and one profile was dominant by both typing methods. In contrast, PFGE had very high discriminatory power, demonstrating 35 profiles. On the basis of PFGE patterns, a similarity matrix and a dendrogram were constructed. The results indicated that Scandinavian strains and southern European isolates (with some exceptions) belong to two different clonal lineages. A few strains from the United States and United Kingdom deviated considerably from each other and from Scandinavian and southern European strains.
摘要
总共研究了 75 株鳗弧菌 O1 血清群菌株，以确定其质粒含量、核糖体分型和脉冲场凝胶电泳（PFGE）图谱。结果显示有 8 种质粒图谱和 6 种核糖体分型，其中一种图谱在两种分型方法中均占优势。相比之下，PFGE 具有非常高的分辨力，显示出 35 种图谱。基于 PFGE 图谱构建了相似性矩阵和聚类树。结果表明，斯堪的纳维亚菌株和南欧分离株（有一些例外）属于两个不同的克隆谱系。来自美国和英国的少数菌株彼此之间以及与斯堪的纳维亚和南欧菌株之间差异较大。
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