Shirasawa H, Tanzawa H, Matsunaga T, Simizu B
Department of Microbiology, School of Medicine, Chiba University, Japan.
Virology. 1991 Oct;184(2):795-8. doi: 10.1016/0042-6822(91)90455-k.
The splicing patterns of E6-E7 transcripts of human papillomavirus type 16 (HPV16) in cervical premalignant lesions were quantitatively analyzed by S1 nuclease protection assay. The major E6-E7 transcripts in HPV16-containing cervical lesions (four cervical intraepithelial neoplasias and one invasive carcinoma) were from spliced E6I/E7 mRNA. The unspliced E6/E7 mRNA, which can encode the full-length zinc finger protein E6, is expressed as 8 to 15% of E6-E7 transcripts. The spliced E6II/E7 mRNAs were expressed as 14 to 24% of E6-E7 transcripts in most tissues. However, in HPV 16-containing cell lines, the expression levels of spliced and unspliced E6-E7 transcripts were variable.
