木聚糖酶 IV，气单胞菌 ME-1 的一种外切木聚糖酶，可将木四糖作为唯一的低分子量寡糖从木聚糖中产生。
Xylanase IV, an Exoxylanase of Aeromonas caviae ME-1 Which Produces Xylotetraose as the Only Low-Molecular-Weight Oligosaccharide from Xylan.
出版信息
Appl Environ Microbiol. 1995 Apr;61(4):1666-8. doi: 10.1128/aem.61.4.1666-1668.1995.
Abstract
A novel xylanase (xylanase IV) which produces xylotetraose as the only low-molecular-weight oligosaccharide from oat spelt xylan was isolated from the culture medium of Aeromonas caviae ME-1. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the xylanase IV molecular weight was 41,000. Xylanase IV catalyzed the hydrolysis of oat spelt xylan, producing exclusively xylotetraose. The acid hydrolysate of the product gave d-xylose. The enzyme did not hydrolyze either p-nitrophenyl-(beta)-d-xyloside, small oligosaccharides (xylobiose and xylotetraose), or polysaccharides, such as starch, cellulose, carboxymethyl cellulose, laminarin, and (beta)-1,3-xylan.
摘要
从豚鼠气单胞菌 ME-1 的培养液中分离到一种新型木聚糖酶（木聚糖酶 IV），它能将燕麦 spelt 木聚糖水解成唯一的低分子量寡糖——木四糖。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，木聚糖酶 IV 的分子量为 41000。木聚糖酶 IV 能催化燕麦 spelt 木聚糖水解，只产生木四糖。产物的酸水解产物为 D-木糖。该酶不能水解对硝基苯基-β-D-木糖苷、小寡糖（木二糖和木四糖）或多糖，如淀粉、纤维素、羧甲基纤维素、昆布多糖和β-1,3-木聚糖。
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