Appl Environ Microbiol. 1995 Nov;61(11):3865-71. doi: 10.1128/aem.61.11.3865-3871.1995.
Four detergents were tested as selective C sources for the plant growth-promoting rhizobacteria Pseudomonas aeruginosa 7NSK2 and Pseudomonas fluorescens ANP15. CO-720 (Igepal CO-720) or DOS (dioctyl sulfosuccinate), applied at 0.2% to the soil, increased the number of detergent-adapted, inoculated strains by almost 1.5 log units after 25 days, accounting for virtually the entire increase in total bacteria. The same dose of Tween 80 or N-laurylsarcosine, on the other hand, increased the indigenous populations by almost 2.5 log units, with only minor increases in the number of detergent-adapted inoculated strains. When CO-720 or DOS was initially supplied, the number of detergent-adapted 7NSK2 organisms was about 2 log units higher after 3 months of incubation than for the detergent-unadapted strain. This better survival resulted in a significantly higher root colonization of maize in a pot experiment with soil inoculation, with a significantly (P <= 0.05) higher shoot dry weight (18 to 33%). In a first field experiment with rhizosphere inoculation of 1-month-old maize plants, no effects on the height of two maize cultivars could be observed 1 month after inoculation. In a second field experiment, leaf and stem dry weights of yellow mustard and grass dry weight were increased in the treatments with seed and soil inoculation of the detergent-adapted 7NSK2 in combination with CO-720 application by, respectively, 7 to 8%, 19 to 23%, and 20 to 31%, although only the increases in grass dry weight were statistically significant at P <= 0.1. To some extent, 7NSK2 and DOS application also positively affected the mineral content of yellow mustard.
四种清洁剂被测试为植物促生根际细菌铜绿假单胞菌 7NSK2 和荧光假单胞菌 ANP15 的选择性 C 源。CO-720（伊格尔 CO-720）或 DOS（二辛基琥珀酸磺酸钠）以 0.2%的浓度添加到土壤中，在 25 天后，使适应清洁剂的接种菌株数量增加了近 1.5 个对数单位，几乎占总细菌数量的全部增加。另一方面，吐温 80 或 N-月桂酰肌氨酸的相同剂量使土著种群增加了近 2.5 个对数单位，而适应清洁剂的接种菌株数量仅略有增加。当最初供应 CO-720 或 DOS 时，经过 3 个月的孵育，适应清洁剂的 7NSK2 生物体的数量比未适应清洁剂的菌株高约 2 个对数单位。这种更好的生存导致在土壤接种的盆栽实验中，玉米的根部定植明显增加，地上部分干重（18 至 33%）显著增加（P<=0.05）。在第一个根际接种的田间试验中，接种 1 个月大的玉米植株后，在接种后 1 个月，两个玉米品种的高度没有观察到影响。在第二个田间试验中，通过种子和土壤接种适应清洁剂的 7NSK2 并结合 CO-720 应用，黄芥菜的叶片和茎干重以及草的干重分别增加了 7%至 8%、19%至 23%和 20%至 31%，尽管只有草的干重增加在 P<=0.1 时具有统计学意义。在某种程度上，7NSK2 和 DOS 的应用也对黄芥菜的矿物质含量产生了积极影响。
