利用 4-硝基苯氧基乙酸检测和定量 2,4-二氯苯氧乙酸（2,4-D）/α-酮戊二酸双加氧酶活性在 2,4-D 降解微生物中的应用。
Use of 4-Nitrophenoxyacetic Acid for Detection and Quantification of 2,4-Dichlorophenoxyacetic Acid (2,4-D)/(alpha)-Ketoglutarate Dioxygenase Activity in 2,4-D-Degrading Microorganisms.
出版信息
Appl Environ Microbiol. 1997 Mar;63(3):1189-91. doi: 10.1128/aem.63.3.1189-1191.1997.
Abstract
Purified 2,4-dichlorophenoxyacetic acid (2,4-D)/(alpha)-ketoglutarate dioxygenase (TfdA) was shown to use 4-nitrophenoxyacetic acid (K(infm) = 0.89 (plusmn) 0.04 mM, k(infcat) [catalytic constant] = 540 (plusmn) 10 min(sup-1)), producing intensely yellow 4-nitrophenol. This reagent was used to develop a rapid, continuous, colorimetric assay for the detection of TfdA and analogous activities in 2,4-D-degrading bacterial cells and extracts.
摘要
已显示，经纯化的 2,4-二氯苯氧乙酸（2,4-D）/α-酮戊二酸双加氧酶（TfdA）能利用 4-硝基苯氧乙酸（K(infm) = 0.89（plusmn）0.04 mM，k(infcat) [催化常数] = 540（plusmn）10 min(sup-1)），生成深黄色的 4-硝基苯酚。该试剂被用于开发一种快速、连续的比色测定法，以检测 2,4-D 降解细菌细胞和提取物中的 TfdA 及类似活性。
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