孵育温度对多氯联苯（PCB）污染和无 PCB 淡水沉积物中微生物还原脱氯 2,3,4,6-四氯联苯途径的影响。
Effect of Incubation Temperature on the Route of Microbial Reductive Dechlorination of 2,3,4,6-Tetrachlorobiphenyl in Polychlorinated Biphenyl (PCB)-Contaminated and PCB-Free Freshwater Sediments.
出版信息
Appl Environ Microbiol. 1997 Jul;63(7):2836-43. doi: 10.1128/aem.63.7.2836-2843.1997.
We studied the influence of temperature (4 to 66(deg)C) on the microbial dechlorination of 2,3,4,6-tetrachlorobiphenyl (2,3,4,6-CB) incubated for 1 year in anaerobic sediments from Woods Pond in Lenox, Mass., and Sandy Creek Nature Center Pond (SCNC) in Athens, Ga. Seven discrete dechlorination reactions were observed, four of which occurred in both sediments. These were 2,3,4,6-CB (symbl) 2,4,6-CB, 2,3,4,6-CB (symbl) 2,3,6-CB, 2,4,6-CB (symbl) 2,6-CB, and 2,3,6-CB (symbl) 2,6-CB. Three additional reactions occurred only in Woods Pond sediment. These were 2,4,6-CB (symbl) 2,4-CB, 2,4-CB (symbl) 2-CB, and 2,4-CB (symbl) 4-CB. The dechlorination reactions exhibited at least four different temperature dependencies in SCNC sediment and at least six in Woods Pond sediment. We attribute the discrete dechlorination reactions to different polychlorinated biphenyl (PCB)-dechlorinating microorganisms with distinct specificities. Temperature influenced the timing and the relative predominance of parallel pathways of dechlorination, i.e., meta versus para dechlorination of 2,3,4,6-CB and ortho versus para dechlorination of 2,4,6-CB and 2,4-CB. meta dechlorination of 2,3,4,6-CB to 2,4,6-CB dominated at all tested temperatures except at 18 and 34(deg)C, where para dechlorination to 2,3,6-CB dominated in some replicates. The dechlorination of 2,4,6-CB was restricted to (symbl)15 to 30(deg)C in both sediments. Temperature affected the lag time preceding the dechlorination of 2,4,6-CB in both sediments and affected the preferred route of its dechlorination in Woods Pond sediment. para dechlorination dominated at 20(deg)C, and ortho dechlorination dominated at 15(deg)C, but at 18 and 22 to 30(deg)C the relative dominance of ortho versus para dechlorination of 2,4,6-CB varied. These data indicate that field temperatures play a significant role in controlling the nature and the extent of the PCB dechlorination that occurs at a given site.
我们研究了温度（4 至 66°C）对 2,3,4,6-四氯联苯（2,3,4,6-CB）微生物脱氯的影响，这些 2,3,4,6-CB 是在马萨诸塞州莱诺克斯的伍兹池塘和佐治亚州雅典的桑迪溪自然中心池塘的厌氧沉积物中培养了 1 年的。观察到了七种不同的脱氯反应，其中四种发生在两种沉积物中。这些是 2,3,4,6-CB（symbl）2,4,6-CB、2,3,4,6-CB（symbl）2,3,6-CB、2,4,6-CB（symbl）2,6-CB 和 2,3,6-CB（symbl）2,6-CB。另外三种反应仅发生在伍兹池塘沉积物中。这些是 2,4,6-CB（symbl）2,4-CB、2,4-CB（symbl）2-CB 和 2,4-CB（symbl）4-CB。在 SCNC 沉积物中，脱氯反应表现出至少四种不同的温度依赖性，在伍兹池塘沉积物中表现出至少六种温度依赖性。我们将离散的脱氯反应归因于具有不同特异性的不同多氯联苯（PCB）脱氯微生物。温度影响脱氯的时间和同时发生的脱氯途径的相对优势，即 2,3,4,6-CB 的间位脱氯和对位脱氯以及 2,4,6-CB 和 2,4-CB 的邻位脱氯。在所有测试温度下，除了在 18 和 34°C 下某些重复实验中 2,3,4,6-CB 的对位脱氯占主导地位外，2,3,4,6-CB 到 2,4,6-CB 的间位脱氯占主导地位。在两种沉积物中，2,4,6-CB 的脱氯被限制在 15 至 30°C。温度影响了两种沉积物中 2,4,6-CB 脱氯前的滞后时间，并影响了其在伍兹池塘沉积物中的脱氯首选途径。在 20°C 时，对位脱氯占主导地位，在 15°C 时，邻位脱氯占主导地位，但在 18 和 22 至 30°C 时，2,4,6-CB 的邻位脱氯和对位脱氯的相对优势发生了变化。这些数据表明，现场温度在控制特定地点发生的 PCB 脱氯的性质和程度方面起着重要作用。
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