Appl Environ Microbiol. 1997 Oct;63(10):3804-9. doi: 10.1128/aem.63.10.3804-3809.1997.
The ability of cellobiose dehydrogenase purified from Phanerochaete chrysosporium to modify a Douglas fir kraft pulp was assessed. Although the addition of cellobiose dehydrogenase alone had little effect, supplementation with cellobiose and iron resulted in a substantial reduction in the degree of polymerization of the pulp cellulose. When the reaction was monitored over time, a progressive depolymerization of the cellulose was apparent with the concomitant production of cellobiono-1,5-lactone. Analysis of the reaction filtrates indicated that glucose and arabinose were the only neutral sugars generated. These sugars are derived from the degradation of the cellobiose rather than resulting from modifications of the pulp. These results suggest that the action of cellobiose dehydrogenase results in the generation of hydroxyl radicals via Fenton's chemistry which subsequently results in the depolymerization of cellulose. This appears to be the mechanism whereby a substantial reduction in the degree of polymerization of the cellulose can be achieved without a significant release of sugar.
评估了从黄孢原毛平革菌中纯化得到的纤维二糖脱氢酶修饰花旗松牛皮纸浆的能力。尽管单独添加纤维二糖脱氢酶几乎没有效果，但补充纤维二糖和铁会导致纸浆纤维素的聚合度显著降低。随着时间的推移监测反应时，纤维素的解聚是明显的，同时产生了纤维二糖-1,5-内酯。对反应滤液的分析表明，只有中性糖生成了葡萄糖和阿拉伯糖。这些糖是从纤维二糖的降解中产生的，而不是来自纸浆的修饰。这些结果表明，纤维二糖脱氢酶的作用通过芬顿化学产生了羟基自由基，随后导致纤维素的解聚。这似乎是在不显著释放糖的情况下实现纤维素聚合度大幅降低的机制。
