Takahashi K, Sato K
Department of Oral Surgery, School of Medicine, Chiba University.
Hum Cell. 1991 Mar;4(1):51-7.
A human malignant fibrous histiocytoma (MFH) cell line, designated as MFH-ino, was established from the maxillary tumor of a 45-year-old woman. Clinically, the original tumor was accompanied by extensive destruction of the surrounding tissues. Cells were obtained from the explant culture of tumor fragments. Both histiocytic and fibroblastic markers were observed in the histochemical and immunocytochemical studies of MFH-ino. The cells were positive for lysozyme, alpha-1-antichymotrypsin, and the collagen types I, III, IV, V, but were negative for alpha-1-antitrypsin, acetate esterase and type II collagen. As biochemical examinations of the culture cells, collagen synthesis was assayed by the measurement of hydroxyproline and the content increase in culture dishes with time after cell inoculation. Collagenase activity secreted in culture medium was also examined with FITC-labeled type I collagen as substrate, and high activity was detected at the late stage of the stationary phase. Further, the MFH-ino cells had high acid phosphatase activity while lacking alkaline phosphatase activity. These findings indicated that MFH-ino cells expressed the various properties of MFH, which will be of importance for understanding the biological behavior, and especially the collagen metabolism, of MFH.
从一名45岁女性上颌肿瘤中建立了一株人恶性纤维组织细胞瘤（MFH）细胞系，命名为MFH-ino。临床上，原发肿瘤伴有周围组织的广泛破坏。细胞取自肿瘤碎片的外植体培养。在对MFH-ino的组织化学和免疫细胞化学研究中观察到了组织细胞和成纤维细胞标志物。这些细胞对溶菌酶、α-1抗糜蛋白酶以及I、III、IV、V型胶原呈阳性反应，但对α-1抗胰蛋白酶、醋酸酯酶和II型胶原呈阴性反应。作为对培养细胞的生化检测，通过测量羟脯氨酸来测定胶原合成，并在细胞接种后随时间观察培养皿中含量的增加。还用异硫氰酸荧光素标记的I型胶原作为底物检测了培养基中分泌的胶原酶活性，在静止期后期检测到高活性。此外，MFH-ino细胞具有高酸性磷酸酶活性，而缺乏碱性磷酸酶活性。这些发现表明MFH-ino细胞表现出MFH的各种特性，这对于理解MFH的生物学行为，尤其是胶原代谢具有重要意义。
