Venter Andre, Makgwane Peter R, Rohwer Egmont R
Chemistry Department, University of Pretoria, Pretoria, South Africa.
Anal Chem. 2006 Mar 15;78(6):2051-4. doi: 10.1021/ac051693a.
A porous layer open tubular (PLOT) silica gel column was used together with subcritical CO2 as the mobile phase to effect the group separation of polar oxygenated compounds. Aliphatic and aromatic compounds were shown to elute together. This group was followed by ethers and aldehydes, which were separated from compounds containing an alcohol functional group. Compounds with a carboxylic acid moiety could also be eluted from the silica gel. The group separation obtained when a silica gel PLOT column is used together with subcritical CO2 was also demonstrated to be valuable as the first dimension of a comprehensive two-dimensional SFCxGC analysis where the GC analysis in the second dimension is performed with a fast and independently heated temperature programmed gas chromatograph. With this combination of SFC and GC, many of the oxygenated compounds, routinely found in petroleum samples, could successfully be separated and identified.
使用多孔层开管（PLOT）硅胶柱，并以亚临界二氧化碳作为流动相，以实现极性含氧化合物的族分离。脂肪族和芳香族化合物显示会一起洗脱。随后是醚类和醛类，它们与含有醇官能团的化合物分离。含有羧酸部分的化合物也可以从硅胶上洗脱下来。当硅胶PLOT柱与亚临界二氧化碳一起使用时获得的族分离，也被证明作为全二维超临界流体色谱-气相色谱（SFCxGC）分析的第一维是有价值的，其中第二维的气相色谱分析是使用快速且独立加热的程序升温气相色谱仪进行的。通过这种超临界流体色谱和气相色谱的组合，石油样品中经常发现的许多含氧化合物能够成功地分离和鉴定。
