Boreskov Institute of Catalysis, pr. Lavrentieva 5, Novosibirsk, 630090, Russia; Novosibirsk State University, Pirogova Str., 2, Novosibirsk, 630090, Russia.
Boreskov Institute of Catalysis, pr. Lavrentieva 5, Novosibirsk, 630090, Russia.
J Chromatogr A. 2018 Dec 7;1579:83-88. doi: 10.1016/j.chroma.2018.10.015. Epub 2018 Oct 10.
Up to the present, comprehensive two-dimensional gas chromatography was commonly performed using combinations of columns with stationary liquid phases. There are singular examples with a porous layer open tubular (PLOT) column used only in the second dimension. However, GC × GC systems with two PLOT columns are not reported in the literature. Our work describes the application of two PLOT columns in the GC × GC mode. In the first and second dimensions we used columns with the sorbents based on organic porous polymers with different selectivity: Rt-Q-BOND (nonpolar column consist of 100% divinylbenzene-styrene), Rt-S-BOND (intermediate polar column, which contain 4-vinylpyridine), Rt-U-BOND (polar column based on divinylbenzene - ethylene glycol dimethylacrylate copolymer), DVB-VIm (divinylbenzene-vinylimidazole copolymer), a column PTMSP with poly-(1-trimethylsilyl-1-propyne) sorbent, and a GASPRO column with porous silica as a stationary phase. The degree of orthogonality was calculated for five different combinations of columns, where a column with the porous polymer was used in the first dimension, and GASPRO column - in the second dimension. Orthogonality was estimated from the correlation coefficient of retention times in the first and second dimensions. Examples of the separation of C-C hydrocarbons on a combination of PTMSP - GASPRO and Rt-Q-BOND - GASPRO columns are reported. It is shown that not only light hydrocarbons but also compounds belonging to different chemical classes can be separated with the use of PLOT columns.
到目前为止，综合二维气相色谱法通常使用固定相的柱组合来进行。虽然有一个单独的例子是仅在第二维中使用多孔层开放管（PLOT）柱。然而，文献中没有报道过具有两个 PLOT 柱的 GC×GC 系统。我们的工作描述了在 GC×GC 模式下使用两个 PLOT 柱的应用。在第一维和第二维中，我们使用了基于具有不同选择性的有机多孔聚合物的吸附剂的柱：Rt-Q-BOND（由 100%二乙烯基苯-苯乙烯组成的非极性柱）、Rt-S-BOND（含有 4-乙烯基吡啶的中等极性柱）、Rt-U-BOND（基于二乙烯基苯-乙二醇二甲基丙烯酸酯共聚物的极性柱）、DVB-VIm（二乙烯基苯-乙烯基咪唑共聚物）、带有聚（1-三甲基硅基-1-丙炔）吸附剂的 PTMSP 柱和多孔硅胶作为固定相的 GASPRO 柱。对于五种不同柱组合计算了正交度，其中在第一维中使用多孔聚合物柱，在第二维中使用 GASPRO 柱。正交度是从第一维和第二维中保留时间的相关系数估计的。报道了在 PTMSP-GASPRO 和 Rt-Q-BOND-GASPRO 柱组合上分离 C-C 烃的实例。结果表明，不仅可以分离轻烃，而且还可以使用 PLOT 柱分离属于不同化学类别的化合物。
