De Herdt P, Jagt E, Paul G, Van Colen S, Renard R, Destrooper C, van den Bosch G
Intervet Belgium, Bedrijvenlaan 7, 2800, Mechelen, Belgium.
Avian Pathol. 2005 Dec;34(6):501-4. doi: 10.1080/03079450500368417.
In order to evaluate the usefulness of the enzyme-linked immunosorbent assay (ELISA) in determining the optimal age for infectious bursal disease virus (IBDV) vaccination in broilers under field circumstances, the percentage of birds susceptible to vaccine at vaccination age was estimated in 12 flocks on the basis of ELISA titres in newborn chicks. The effective percentage of susceptible birds at the day of vaccination was checked by comparing virus neutralization (VN) titres measured in sera taken immediately before vaccine administration with the breakthrough titre of the vaccine used. In nine of the 12 flocks, the ELISA had largely overestimated the percentage of susceptible birds. Subsequently, to examine the performance of the ELISA kit, 815 chicken sera were comparatively examined in ELISA and VN tests. Results demonstrated a linear relationship between mean ELISA titres and VN titres. It was concluded that the ELISA could be a useful tool in IBDV vaccination timing, but that interpretation criteria should be adjusted to avoid vaccination at too early an age.
为了评估酶联免疫吸附测定（ELISA）在确定田间条件下肉鸡传染性法氏囊病病毒（IBDV）最佳免疫日龄方面的实用性，根据新生雏鸡的ELISA效价，对12个鸡群中免疫日龄时对疫苗敏感的鸡的百分比进行了估算。通过比较疫苗接种前立即采集的血清中测得的病毒中和（VN）效价与所用疫苗的突破效价，来检查免疫日当天易感鸡的有效百分比。在12个鸡群中的9个鸡群中，ELISA大大高估了易感鸡的百分比。随后，为了检测ELISA试剂盒的性能，对815份鸡血清进行了ELISA和VN试验的比较检测。结果表明，平均ELISA效价与VN效价之间存在线性关系。得出的结论是，ELISA可能是IBDV免疫接种时机选择的一个有用工具，但应调整判读标准以避免过早免疫。
