Wang Baode, Gao Ruitong, Mastro Victor C, Reardon Richard C
USDA-APHIS-PPQ-CPHST, Otis Pest Survey, Detection and Exclusion Laboratory, Bldg. 1398, Otis ANGB, MA 02542, USA.
J Econ Entomol. 2005 Dec;98(6):2292-300. doi: 10.1603/0022-0493-98.6.2292.
As part of the ongoing evaluation of different systemic insecticides for prophylactic treatment of trees, responses of the beetle Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae) to different doses of four systemic neonicotinyl insecticides were studied. Adult beetles were provided with twigs or leaves of trees treated with different concentrations of imidacloprid to evaluate the toxicity of the insecticide through ingestion or contact or through both. Adult beetles also were provided with twigs of host plant treated with clothianidin, dinotefuran, and thiamethoxam to establish dose response of the beetle to these insecticides. Levels of individual insecticides in twigs and leaves were determined by using the "parent" method with high-performance liquid chromatography, and these levels were compared with the applied concentrations to determine their relationship. The LC50 values for detected level of each insecticide in twigs was 5.1 ppm at 24 h, 2.9 at 48 h, and 1.9 ppm at 72 h for imidacloprid; 1.1 ppm at 72 h for clothianidin; 2.2 ppm at 72 h for dinotefuran; and 1.0 ppm at 72 h for thiamethoxam. Our results indicate that mortality of adult beetles resulted not only from the ingestion and contact toxicity but also possibly from the antifeedant effect of imidacloprid.
作为对不同系统性杀虫剂用于树木预防性处理的持续评估的一部分，研究了光肩星天牛（Anoplophora glabripennis (Motschulsky)）（鞘翅目：天牛科）对四种系统性新烟碱类杀虫剂不同剂量的反应。为成虫提供用不同浓度吡虫啉处理过的树枝或树叶，以通过摄入、接触或两者兼有的方式评估杀虫剂的毒性。还为成虫提供用噻虫胺、呋虫胺和噻虫嗪处理过的寄主植物树枝，以确定该甲虫对这些杀虫剂的剂量反应。使用高效液相色谱的“母体”方法测定树枝和树叶中各杀虫剂的含量，并将这些含量与施用浓度进行比较以确定它们之间的关系。吡虫啉在树枝中检测到的水平的LC50值在24小时时为5.1 ppm，48小时时为2.9 ppm，72小时时为1.9 ppm；噻虫胺在72小时时为1.1 ppm；呋虫胺在72小时时为2.2 ppm；噻虫嗪在72小时时为1.0 ppm。我们的结果表明，成虫的死亡不仅是由于摄入和接触毒性，还可能是由于吡虫啉的拒食作用。
