Krueger G R, Sander C, Hoffmann A, Barth A, Koch B, Braun M
Immunopathology Laboratory, University of Cologne, F.R.G.
In Vivo. 1991 May-Jun;5(3):217-25.
The prevalence and activity of human herpesvirus-6 in patients with collagen vascular diseases (CVD) was determined. One hundred and fifty patients with CVD (56 with systemic lupus erythematosus-SLE, 92 with rheumatoid arthritis-RA, 1 with Sharp's syndrome and 1 with atypical polyclonal lymphoproliferation-APL and rheumatoid features) were screened serologically (IFA and ELISA) for antibodies against human herpesvirus-6 (HHV-6), Epstein-Barr virus (EBV) and cytomegalovirus (CMV). Virus isolation was attempted from peripheral blood lymphocytes (PBL) of 25 persons with various disorders. PBL were grown in tissue culture and tested with standard HHV-6-positive antisera for viral antigen expression. Supernatants of the patient's lymphocyte cultures were used to infect HSB2 cells, and virus infection in these cells was proven by IFA, in situ hybridization and by electron microscopy. Fifty-five percent of the SLE patients, 6.5% of the RA patients and both patients with Sharp's syndrome or with APL had antibody titers indicative of active HHV-6 infection. Virus cultures were positive in 9 of the 25 attempts with establishment of stable virus lines. These patients were 5 with SLE or UCVD, and one each with RA, CFS, APL as well as one healthy control. Reactivated and chronic active HHV-6 infections are frequent in SLE like EBV in RA. The role of these viruses in the pathogenesis of the diseases or in their reactivation still needs further investigation.
测定了胶原血管疾病（CVD）患者中人类疱疹病毒6型（HHV-6）的患病率和活性。对150例CVD患者（56例系统性红斑狼疮-SLE、92例类风湿关节炎-RA、1例夏普综合征患者和1例具有类风湿特征的非典型多克隆淋巴细胞增殖-APL患者）进行了血清学筛查（免疫荧光分析和酶联免疫吸附测定），以检测抗人类疱疹病毒6型（HHV-6）、爱泼斯坦-巴尔病毒（EBV）和巨细胞病毒（CMV）的抗体。尝试从25例患有各种疾病的患者的外周血淋巴细胞（PBL）中分离病毒。将PBL在组织培养中培养，并用标准的HHV-6阳性抗血清检测病毒抗原表达。患者淋巴细胞培养物的上清液用于感染HSB2细胞，通过免疫荧光分析、原位杂交和电子显微镜证实这些细胞中的病毒感染。55%的SLE患者、6.5%的RA患者以及夏普综合征或APL患者的抗体滴度均表明存在活跃的HHV-6感染。在25次尝试中有9次病毒培养呈阳性，并建立了稳定的病毒株系。这些患者包括5例SLE或未分化结缔组织病（UCVD）患者，以及1例RA、慢性疲劳综合征（CFS）、APL患者和1例健康对照者。与RA中EBV一样，SLE中重新激活的和慢性活跃的HHV-6感染很常见。这些病毒在疾病发病机制或其重新激活中的作用仍需进一步研究。
