Murayama Tsugiya, Yamaguchi Nobuo, Iwamoto Kozo, Eizuru Yoshito
Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Antivir Chem Chemother. 2006;17(1):11-6. doi: 10.1177/095632020601700102.
We examined the effect of Kampo on the replication of ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) in the human embryonic fibroblast cell line MRC-5. Treatment of HCMV-infected cells with Sho-seiryu-to (SST; Xiao-Qing-Long-Tang in Chinese) resulted in the inhibition of viral replication without affecting the cell growth. SST treatment decreased the synthesis of viral DNA, but had no virucidal effect on cell-free HCMV. However, the inhibitory effect of SST on HCMV replication was ablated by anti-interferon-beta (IFN-beta) antibody suggesting that SST inhibits the replication of GCV-resistant HCMV through the induction of IFN-beta. These results suggest that SST is a novel compund with potential as an anti-HCMV.
我们研究了汉方药对耐更昔洛韦（GCV）的人巨细胞病毒（HCMV）在人胚肺成纤维细胞系MRC-5中复制的影响。用小青龙汤（SST；中文为小青龙汤）处理HCMV感染的细胞可抑制病毒复制，且不影响细胞生长。SST处理可减少病毒DNA的合成，但对游离的HCMV无杀病毒作用。然而，抗β干扰素（IFN-β）抗体消除了SST对HCMV复制的抑制作用，这表明SST通过诱导IFN-β来抑制耐GCV的HCMV的复制。这些结果表明，SST是一种具有抗HCMV潜力的新型化合物。
