Riollet Céline, Mutuel Doriane, Duonor-Cérutti Martine, Rainard Pascal
Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
J Interferon Cytokine Res. 2006 Mar;26(3):141-9. doi: 10.1089/jir.2006.26.141.
Interleukin-17 (IL-17) is a proinflammatory cytokine produced by activated memory T cells, and it appears to play an upstream role in T cell-triggered inflammation by stimulating stromal cells to secrete other cytokines. We hypothesize that IL-17 plays a role in the recruitment of neutrophils in the bovine mammary gland during infection or immune-mediated inflammation. The rapid amplification of cDNA ends (RACE) method was used to obtain a cDNA of bovine IL-17 (BoIL-17) containing a 462-bp open reading frame (ORF) encoding a protein of 153 amino acids (aa) with a molecular mass of 17.2 kDa, a 23-residue NH(2)-terminal signal peptide, a single potential N-linked glycosylation site, and 6 cysteine residues. BoIL-17 protein shared 73.5% identity with the human protein and 67% with the mouse and rat proteins. Sf9 insect cells were transfected with BoIL-17 cDNA, and supernatant was tested for biologic activity on a primary culture of bovine mammary epithelial cells (MECs). mRNA synthesis of IL-6, IL-8, and growth-related oncogene alpha (Groalpha) was induced, suggesting a functional role for IL-17 in mammary immunity.
白细胞介素-17（IL-17）是一种由活化的记忆T细胞产生的促炎细胞因子，它似乎通过刺激基质细胞分泌其他细胞因子，在T细胞引发的炎症中发挥上游作用。我们推测，IL-17在牛乳腺感染或免疫介导的炎症过程中，对中性粒细胞的募集起作用。采用cDNA末端快速扩增（RACE）方法获得了牛IL-17（BoIL-17）的cDNA，其开放阅读框（ORF）为462 bp，编码一个153个氨基酸（aa）的蛋白质，分子量为17.2 kDa，有一个23个残基的NH(2)-末端信号肽、一个潜在的N-连接糖基化位点和6个半胱氨酸残基。BoIL-17蛋白与人类蛋白的同源性为73.5%，与小鼠和大鼠蛋白的同源性为67%。用BoIL-17 cDNA转染Sf9昆虫细胞，并检测其对牛乳腺上皮细胞（MECs）原代培养物上清液的生物学活性。结果诱导了IL-6、IL-8和生长相关癌基因α（Groα）的mRNA合成，提示IL-17在乳腺免疫中发挥功能作用。
