Liu Huaipan, Ji Xiu'e, Yu Bingjun, Liu Youliang
Ministry of Agriculture Key Laboratory of Crop Growth Regulation, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Sci China C Life Sci. 2006 Feb;49(1):12-7. doi: 10.1007/s11427-004-5084-y.
The contents of covalently conjugated polyamines (CC-PAs) and noncovalently conjugated polyamines (NCC-PAs) to deoxyribonucleic acid-protein (DNP) isolated from wheat (Triticum aestivum L.) seedling roots under osmotic stress were detected. Results showed thatafter osmotic stress treatment for 7 d, the levels in NCC-spermine (NCC-Spm) and NCC-spermidine (NCC-Spd) of drought-tolerant Yumai No. 18 cv. increased more markedly than that of drought-sensitive Yangmai No. 9 cv., while the NCC-putrescine (NCC-Put) could not be statistically detected in two cultivars. Exogenous Spm treatment alleviated osmotic stress injury to Yangmai No. 9 cv. seedlings, coupled with marked increases of NCC-Spm and NCC-Spd levels of this cultivar. Under PEG osmotic stress, the concomitant treatment of drought-tolerant Yumai No. 18 cv.seedlings with methylglyoxyl-bis (guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), aggravated osmotic stress injury to this cultivar, coupled with market decreases of the NCC-Spm and NCC-Spd levels. The levels in CC-Put and CC-Spd of drought-tolerant Yumai No. 18 cv. increased more markedly than that of drought-sensitive Yangmai No. 9 cv. Under osmotic stress. The treatment of drought-tolerant Yumai No. 18 cv. seedlings with phenanthrolin (o-Phen), an inhibitor of transglutaminase (TGase), aggravated osmotic stress injury to this cultivar, coupled with a reduction of sum contents of CC-Put+CC-Spd. These results suggested that NCC-Spm and NCC-Spd, together with CC-Put and CC-Spd, in DNP of roots could enhance tolerance of the wheat seedlings to osmotic stress.
检测了渗透胁迫下从小麦（普通小麦）幼苗根系中分离出的与脱氧核糖核酸 - 蛋白质（DNP）共价结合的多胺（CC - PAs）和非共价结合的多胺（NCC - PAs）的含量。结果表明，渗透胁迫处理7天后，耐旱品种豫麦18号的非共价结合亚精胺（NCC - Spm）和非共价结合亚精脒（NCC - Spd）水平比干旱敏感品种扬麦9号增加得更显著，而两个品种中均未检测到非共价结合腐胺（NCC - Put）的统计学差异。外源亚精胺处理减轻了扬麦9号幼苗的渗透胁迫损伤，同时该品种的NCC - Spm和NCC - Spd水平显著增加。在聚乙二醇渗透胁迫下，耐旱品种豫麦18号幼苗同时用S - 腺苷甲硫氨酸脱羧酶（SAMDC）抑制剂甲基乙二醛 - 双（脒基腙）（MGBG）处理，加重了该品种的渗透胁迫损伤，同时NCC - Spm和NCC - Spd水平显著降低。渗透胁迫下，耐旱品种豫麦18号的共价结合腐胺（CC - Put）和共价结合亚精脒（CC - Spd）水平比干旱敏感品种扬麦9号增加得更显著。用转谷氨酰胺酶（TGase）抑制剂菲咯啉（邻菲咯啉，o - Phen）处理耐旱品种豫麦18号幼苗，加重了该品种的渗透胁迫损伤，同时CC - Put + CC - Spd的总含量降低。这些结果表明，根系DNP中的NCC - Spm和NCC - Spd以及CC - Put和CC - Spd可以增强小麦幼苗对渗透胁迫的耐受性。
