Kveberg Lise, Bäck Camilla J, Dai Ke-Zheng, Inngjerdingen Marit, Rolstad Bent, Ryan James C, Vaage John T, Naper Christian
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Norway;
J Immunol. 2006 Apr 1;176(7):4133-40. doi: 10.4049/jimmunol.176.7.4133.
The proximal region of the NK gene complex encodes the NKR-P1 family of killer cell lectin-like receptors which in mice bind members of the genetically linked C-type lectin-related family, while the distal region encodes Ly49 receptors for polymorphic MHC class I molecules. Although certain members of the NKR-P1 family are expressed by all NK cells, we have identified a novel inhibitory rat NKR-P1 molecule termed NKR-P1C that is selectively expressed by a Ly49-negative NK subset with unique functional characteristics. NKR-P1C(+) NK cells efficiently lyse certain tumor target cells, secrete cytokines upon stimulation, and functionally recognize a nonpolymorphic ligand on Con A-activated lymphoblasts. However, they specifically fail to kill MHC-mismatched lymphoblast target cells. The NKR-P1C(+) NK cell subset also appears earlier during development and shows a tissue distribution distinct from its complementary Ly49s3(+) subset, which expresses a wide range of Ly49 receptors. These data suggest the existence of two major, functionally distinct populations of rat NK cells possessing very different killer cell lectin-like receptor repertoires.
NK基因复合体的近端区域编码杀伤细胞凝集素样受体的NKR-P1家族，在小鼠中该家族可结合遗传连锁的C型凝集素相关家族的成员，而远端区域则编码针对多态性MHC I类分子的Ly49受体。尽管NKR-P1家族的某些成员由所有NK细胞表达，但我们已鉴定出一种新型的抑制性大鼠NKR-P1分子，称为NKR-P1C，它由具有独特功能特征的Ly49阴性NK亚群选择性表达。NKR-P1C(+) NK细胞能有效裂解某些肿瘤靶细胞，受刺激后分泌细胞因子，并在功能上识别伴刀豆球蛋白A激活的淋巴母细胞上的一种非多态性配体。然而，它们特异性地无法杀伤MHC不匹配的淋巴母细胞靶细胞。NKR-P1C(+) NK细胞亚群在发育过程中也更早出现，并且显示出与其互补的Ly49s3(+)亚群不同的组织分布，后者表达多种Ly49受体。这些数据表明存在两种主要的、功能不同的大鼠NK细胞群体，它们拥有非常不同的杀伤细胞凝集素样受体库。
