脂肪酸对T3与α1和β1甲状腺激素受体结合的竞争性抑制作用。

Competitive inhibition of T3 binding to alpha 1 and beta 1 thyroid hormone receptors by fatty acids.

作者信息

van der Klis F R, Schmidt E D, van Beeren H C, Wiersinga W M

机构信息

Division of Endocrinology, Academic Medical Centre, Amsterdam, The Netherlands.

出版信息

Biochem Biophys Res Commun. 1991 Sep 16;179(2):1011-6. doi: 10.1016/0006-291x(91)91919-4.

DOI:10.1016/0006-291x(91)91919-4

PMID:1654895

Abstract

This study was undertaken to investigate whether fatty acids inhibit the binding of T3 to the alpha 1 and beta 1 form of the thyroid hormone receptor. Fatty acids inhibited the binding of T3 to both receptor proteins isolated from a bacterial expression system. The effectiveness of inhibition depends on the chain length and degree of saturation of the fatty acids. The inhibition of T3 binding to the alpha 1 and beta 1 receptor by oleic acid is competitive in nature; the Ki value was 5.4 10(-6) M for the c-erbA alpha 1 protein and 3.3 10(-6) M for the c-erb beta 1 protein. The findings indicate a direct interaction of fatty acids with T3 receptor proteins.

摘要

本研究旨在探讨脂肪酸是否会抑制T3与甲状腺激素受体α1和β1形式的结合。脂肪酸抑制了T3与从细菌表达系统分离出的两种受体蛋白的结合。抑制效果取决于脂肪酸的链长和饱和度。油酸对T3与α1和β1受体结合的抑制本质上是竞争性的；对于c-erbAα1蛋白，Ki值为5.4×10^(-6) M，对于c-erbβ1蛋白，Ki值为3.3×10^(-6) M。这些发现表明脂肪酸与T3受体蛋白之间存在直接相互作用。