一种通过用小鼠载脂蛋白B48免疫“仅载脂蛋白B39”小鼠而产生的、对小鼠载脂蛋白B48和载脂蛋白B100具有特异性的小鼠单克隆抗体。
A mouse monoclonal antibody specific for mouse apoB48 and apoB100 produced by immunizing "apoB39-only" mice with mouse apoB48.
作者信息
Nguyen Anh T, Braschi Sylvie, Geoffrion Michèle, Fong Loren G, Crooke Rosanne M, Graham Mark J, Young Stephen G, Milne Ross
机构信息
Lipoprotein and Atherosclerosis Research Group, University of Ottawa Heart Institute, 40 Ruskin Street, Ottawa, ON, Canada K1Y 4W7.
出版信息
Biochim Biophys Acta. 2006 Feb;1761(2):182-5. doi: 10.1016/j.bbalip.2006.02.004. Epub 2006 Mar 6.
We have generated and characterized a murine monoclonal antibody (mAb) that binds to both mouse apolipoprotein (apo) B48 and apoB100. We immunized "apoB39-only" mice (mice that synthesize a truncated form of apoB, apoB39, but no apoB48 or apoB100) with lipoproteins containing mouse apoB48 and then used splenocytes from the immunized mice to create hybridomas. We identified a hybridoma, 2G11, that secretes a mAb that binds to mouse apoB48 and apoB100 but not to apoB39. Antibody 2G11 also binds apoB48 and apoB100 from rats and hamsters but not from humans. The mAb recognizes mouse apoB equally in very low and low density lipoproteins and was used to quantify apoB in wild-type, apoE-deficient and low-density lipoprotein receptor-deficient mice and in mice treated with an antisense drug that lowers plasma apoB levels. The antibody will be an important reagent for studying mouse models of atherosclerosis. The study also underscores the utility of genetically modified mice for generating mouse mAbs against mouse proteins.
我们制备并鉴定了一种能与小鼠载脂蛋白（apo）B48和apoB100结合的鼠单克隆抗体（mAb）。我们用含有小鼠apoB48的脂蛋白免疫“仅apoB39”小鼠（即合成截短形式的apoB，apoB39，但不合成apoB48或apoB100的小鼠），然后使用免疫小鼠的脾细胞来制备杂交瘤。我们鉴定出一种杂交瘤2G11，它分泌的mAb能与小鼠apoB48和apoB100结合，但不与apoB39结合。抗体2G11也能与大鼠和仓鼠的apoB48和apoB100结合，但不能与人的结合。该mAb在极低密度脂蛋白和低密度脂蛋白中对小鼠apoB的识别能力相同，并被用于定量野生型、载脂蛋白E缺陷型和低密度脂蛋白受体缺陷型小鼠以及用降低血浆apoB水平的反义药物处理的小鼠中的apoB。该抗体将成为研究动脉粥样硬化小鼠模型的重要试剂。这项研究还强调了基因工程小鼠在制备针对小鼠蛋白的鼠单克隆抗体方面的实用性。
Zotero 插件