Imamura T, Tokita Y, Mitsui Y
Cell Science and Technology Division, Fermentation Research Institute, Tsukuba Science City, Japan.
Cell Struct Funct. 1991 Jun;16(3):225-30. doi: 10.1247/csf.16.225.
Modulation of vascular endothelial cell growth by basement membrane heparan sulfate was investigated using four lines of normal and transformed cells. The growth of transformed endothelial cells, but not normal cells, on reconstituted basement membrane was severely suppressed when heparan sulfate, one of the components of the membrane, was specifically degraded by an enzyme, heparitinase. Similarly, when cells were grown on surfaces coated with heparan sulfate, as little as 60 pg/cm2 of heparan sulfate caused growth enhancement of transformed cells, but suppression of normal cells. These results together with our previous observations (IMAMURA, T and MITSUI, Y. (1987) Exp. Cell Res., 172: 92-100) argue that transformed cells have reversed a mechanism by which basement membrane heparan sulfate functions as a physiological suppressor for the growth of normal endothelial cells.
利用四株正常细胞系和转化细胞系研究了基底膜硫酸乙酰肝素对血管内皮细胞生长的调节作用。当膜的成分之一硫酸乙酰肝素被一种酶——乙酰肝素酶特异性降解时，在重组基底膜上转化内皮细胞的生长受到严重抑制，而正常细胞则不受影响。同样，当细胞在涂有硫酸乙酰肝素的表面生长时，低至60 pg/cm2的硫酸乙酰肝素会导致转化细胞的生长增强，但会抑制正常细胞的生长。这些结果与我们之前的观察结果（IMAMURA, T和MITSUI, Y. (1987) Exp. Cell Res., 172: 92 - 100）一起表明，转化细胞已经逆转了基底膜硫酸乙酰肝素作为正常内皮细胞生长的生理抑制剂的作用机制。
