Li Chao, Feng Hong-Chao, Chen Jian-Chao, Song Yu-Feng
Department of Oral and Maxillary Surgery, The Affiliated Hospital, Guiyang Medical College, Guiyang, Guizhou, 550004, PR China.
Ai Zheng. 2005 Nov;24(11):1388-93.
BACKGROUND & OBJECTIVE: Recently, Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) have been identified as potential angiogenic factors. The local balance between them and other angiogenic factors determines the growth, stableness, or regression of blood vessels. This study was to investigate the correlations of Ang-1 and Ang-2 expression to clinicopathologic features, microvessel density (MVD), and vessel maturation index (VMI) of oral squamous cell cacinoma (OSCC), and evaluate the significance of Ang-1 and Ang-2 coexpression in tumor angiogenesis and vessel maturation.
The expression of Ang-1 and Ang-2 in 41 specimens of OSCC, 30 specimens of adjacent noncancerous oral tissues, and 10 specimens of normal oral mucosa was detected by immunohistochemistry. MVD and VMI were assessed with double-labeling immunohistochemical staining against CD34 and alpha-smooth muscle actin (alpha-SMA).
The positive rate of Ang-1 was significantly lower in OSCC than in adjacent noncancerous oral tssues and normal oral mucosa (41.46% vs. 90.00% and 90.0%, P < 0.05); the positive rate of Ang-2 was significantly higher in OSCC than in adjacent noncancerous oral tssues and normal oral mucosa (51.22% vs. 26.67% and 0, P < 0.05). The differences in Ang-1 and Ang-2 expression between adjacent noncancerous oral tssues and normal oral mucosa were not significant (P > 0.05). The positive rate of Ang-1 was significantly higher in highly differentiated tumor than in moderately differentiated tumor (56.00% vs. 18.75%, P < 0.05); the positive rate of Ang-2 was significantly higher in tumor with lymph node metastasis than in tumor without lymph node metastasis (84.62% vs. 35.71%, P < 0.01). Ang-1 and Ang-2 expression were closely correlated with angiogenesis and vessel maturation of tumor (P < 0.05). Ang-1 and Ang-2 had antagonistic effect on the regulation of tumor vessel maturation.
The down-regulation of Ang-1 and up-regulation of Ang-2 are closely related to tumor angiogenesis and vessel maturation.
近来，血管生成素-1（Ang-1）和血管生成素-2（Ang-2）已被确定为潜在的血管生成因子。它们与其他血管生成因子之间的局部平衡决定了血管的生长、稳定性或消退。本研究旨在探讨Ang-1和Ang-2表达与口腔鳞状细胞癌（OSCC）临床病理特征、微血管密度（MVD）及血管成熟指数（VMI）的相关性，并评估Ang-1和Ang-2共表达在肿瘤血管生成和血管成熟中的意义。
采用免疫组织化学法检测41例OSCC标本、30例相邻口腔非癌组织标本及10例正常口腔黏膜标本中Ang-1和Ang-2的表达。采用抗CD34和α-平滑肌肌动蛋白（α-SMA）的双重免疫组织化学染色评估MVD和VMI。
OSCC中Ang-1阳性率显著低于相邻口腔非癌组织和正常口腔黏膜（41.46% 对90.00%和90.0%，P < 0.05）；OSCC中Ang-2阳性率显著高于相邻口腔非癌组织和正常口腔黏膜（51.22% 对26.67%和0，P < 0.05）。相邻口腔非癌组织与正常口腔黏膜之间Ang-1和Ang-2表达差异无统计学意义（P > 0.05）。高分化肿瘤中Ang-1阳性率显著高于中分化肿瘤（56.00% 对18.75%，P < 0.05）；有淋巴结转移的肿瘤中Ang-2阳性率显著高于无淋巴结转移的肿瘤（84.62% 对35.71%，P < 0.01）。Ang-1和Ang-2表达与肿瘤血管生成和血管成熟密切相关（P < 0.05）。Ang-1和Ang-2在调节肿瘤血管成熟方面具有拮抗作用。
Ang-1下调和Ang-2上调与肿瘤血管生成和血管成熟密切相关。
