Xu Guang, Lou Weihua, Sun Zhanwei, Zhang Peng
Department of Otolaryngology, First Hospital of Zhengzhou University, Zhengzhou 450052, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Sep;22(17):772-6.
To investigate the expression of Angiopoietin-1,2 in laryngeal squamous cell carcinoma (LSCC) and the relationship between Angiopoietin-1,2 expression and clinicopathologic parameters and microvessel density (MVD) marked by CD105, and also evaluate the significance of co-expression of Ang-2 and vascular endothelial cell growth factor (VEGF) in tumor angiogenesis.
The expression of Ang-1,2 and VEGF in samples of tumor, para cancer and normal mucosa were detected by immunohistochemical method.
The expression of Angiopoietin-1,2 were identified in LSCC, para cancer tissue and normal mucosa. The VEGF expression was only existed in LSCC. The expression of Ang-1,2 were significantly higher in LSCC than in para cancer tissue (P < 0.05) and normal mucosa (P < 0.01). In the clinicopathologic parameters, only the expression of Ang-2 was closely correlated with clinical stages and MVD (all P < 0.05). There was no significant correlation between the expression of Ang-1,2 and tumor differentiation degree (all P > 0.05). When the expression of Ang-2 and VEGF were both positive, the mean value of MVD was higher than others (P < 0.05).
These results suggest that overexpression of Ang-1,2 may play a very important role in the development of LSCC and are closely correlated with angiogenesis. Ang-2 promote angiogenesis when interacting with VEGF.
探讨血管生成素-1、2在喉鳞状细胞癌（LSCC）中的表达，以及血管生成素-1、2表达与临床病理参数和以CD105标记的微血管密度（MVD）之间的关系，并评估血管生成素-2（Ang-2）与血管内皮生长因子（VEGF）共表达在肿瘤血管生成中的意义。
采用免疫组织化学方法检测肿瘤、癌旁及正常黏膜组织样本中Ang-1、2和VEGF的表达。
在喉鳞状细胞癌、癌旁组织及正常黏膜中均检测到血管生成素-1、2的表达。VEGF表达仅存在于喉鳞状细胞癌中。血管生成素-1、2在喉鳞状细胞癌中的表达显著高于癌旁组织（P<0.05）和正常黏膜（P<0.01）。在临床病理参数中，仅Ang-2的表达与临床分期和MVD密切相关（均P<0.05）。血管生成素-1、2的表达与肿瘤分化程度之间无显著相关性（均P>0.05）。当Ang-2和VEGF表达均为阳性时，MVD的平均值高于其他情况（P<0.05）。
这些结果表明，血管生成素-1、2的过表达可能在喉鳞状细胞癌的发生发展中起非常重要的作用，且与血管生成密切相关。Ang-2与VEGF相互作用时促进血管生成。
