Hendriksen Niels Bohse, Hansen Bjarne Munk
Department of Environmental Chemistry and Microbiology, National Environmental Research Institute, Frederiksborgvej, Roskilde, Denmark.
FEMS Microbiol Lett. 2006 Apr;257(1):106-11. doi: 10.1111/j.1574-6968.2006.00159.x.
The objectives of the study were to develop a specific procedure for quantification and identification of Bacillus thuringiensis kurstaki HD1, which is used as a biopesticide, and to quantify its presence in different kinds of cabbage for human consumption. We found that B. thuringiensis kurstaki HD1 can be distinguished from other B. thuringiensis strains by its unique random amplification of polymophic DNA-PCR pattern with the OPA9 primer and the presence of the flagellin genes, as detected by the primers FLAB1 and FLAB2. We detected from one to 100 Bacillus cereus-like bacteria in 10 batches of five different cabbage products for consumption. As many as 73 out of 134 isolates (53.7%) were identical with B. thuringiensis kurstaki HD1. The results show that B. thuringiensis kurstaki HD1 from biopesticides can be found in vegetables for human consumption.
本研究的目的是开发一种用于定量和鉴定苏云金芽孢杆菌库斯塔克亚种HD1（用作生物农药）的特定程序，并量化其在供人类食用的不同种类卷心菜中的存在情况。我们发现，苏云金芽孢杆菌库斯塔克亚种HD1可通过其使用OPA9引物的独特随机扩增多态性DNA-PCR模式以及引物FLAB1和FLAB2检测到的鞭毛蛋白基因的存在，与其他苏云金芽孢杆菌菌株区分开来。我们在10批5种不同的供食用卷心菜产品中检测到1至100株蜡样芽孢杆菌样细菌。134株分离株中有73株（53.7%）与苏云金芽孢杆菌库斯塔克亚种HD1相同。结果表明，生物农药中的苏云金芽孢杆菌库斯塔克亚种HD1可在供人类食用的蔬菜中被发现。
Zotero 插件