Damgaard P H, Hansen B M, Pedersen J C, Eilenberg J
Department of Ecology and Molecular Biology, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
J Appl Microbiol. 1997 Feb;82(2):253-8.
Bacillus thuringiensis was isolated from the phylloplane of organically grown cabbage in one field during two growth seasons (1992-93). The frequency of B. thuringiensis varied between 0.02 and 0.67 of the total B. cereus/B. thuringiensis population, with an average of 0.11. Characterization of the B. thuringiensis isolates from foliage showed that the majority (64% of 150 isolates) belonged to serovar kurstaki, had bipyramidal crystals and toxicity towards Pieris brassicae and/or Trichoplusia ni. Other serovars were also found on the foliage but occurred at very low frequencies (one to three isolates of each serovar). Bacillus thuringiensis was also isolated from insects associated with the cabbage crop (Pieris rapae (Lep.), Delia radicum (Dip.), Syrphidae ribesii (Dip.) and Aleochara bilineata (Col.)), which were collected alive at different developmental stages in the same field. Serologically these isolates were assigned to the serovars kurstaki, aizawai, tochigiensis, colmeri and indiana/colmeri.
在两个生长季节（1992 - 1993年）期间，从一块田地中有机种植的卷心菜叶面上分离出苏云金芽孢杆菌。苏云金芽孢杆菌在蜡样芽孢杆菌/苏云金芽孢杆菌总菌数中的占比在0.02至0.67之间，平均为0.11。对从叶片上分离出的苏云金芽孢杆菌菌株的特性分析表明，大多数菌株（150个菌株中的64%）属于库尔斯塔克血清型，具有双锥形晶体，对菜粉蝶和/或粉纹夜蛾有毒性。在叶片上也发现了其他血清型，但出现频率极低（每个血清型有一至三个菌株）。苏云金芽孢杆菌还从与卷心菜作物相关的昆虫（菜粉蝶（鳞翅目）、萝卜地种蝇（双翅目）、醋栗花蝇（双翅目）和双线隐翅虫（鞘翅目））中分离得到，这些昆虫是在同一田地的不同发育阶段活体采集的。血清学分析表明，这些分离株属于库尔斯塔克、 aizawai、枥木、科尔默和印第安纳/科尔默血清型。
