Ishizuka T, Yasuda K, Takeda N, Kajita K, Miura K, Nakashima S, Nagao S, Nozawa Y
Third Department of Internal Medicine, Gifu University School of Medicine, Japan.
Endocrinol Jpn. 1991 Feb;38(1):81-7. doi: 10.1507/endocrj1954.38.81.
We have examined thrombin-induced metabolism of phosphoinositides in the platelets from fifteen NIDDM (non-insulin-dependent diabetes mellitus) patients and fifteen healthy subjects (control). The diabetic patients were divided into two groups. One group (group I) had diabetic retinopathy (microangiopathy) and the other group (group II) had atherosclerosis of great vessels (macroangiopathy). In platelets incubated with [32P] orthophosphate for 80 min, the incorporation of 32P radioactivity into phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) was significantly lower in the group II than in the control. The addition of thrombin induced a marked decrease in PIP2 radioactivity at 10 sec in platelets from group I compared with that from the control. These results suggest that the breakdown of polyphosphoinositides is increased in platelets from diabetic subjects with retinopathy, and also that the formation of polyphosphoinositides is decreased in the platelets from diabetic subjects with macroangiopathy.
我们检测了15名非胰岛素依赖型糖尿病（NIDDM）患者和15名健康受试者（对照组）血小板中凝血酶诱导的磷酸肌醇代谢情况。糖尿病患者被分为两组。一组（第一组）患有糖尿病视网膜病变（微血管病变），另一组（第二组）患有大血管动脉粥样硬化（大血管病变）。在用[32P]正磷酸盐孵育80分钟的血小板中，第二组中32P放射性掺入磷脂酰肌醇（PI）、磷脂酰肌醇4-单磷酸（PIP）和磷脂酰肌醇4,5-二磷酸（PIP2）的量显著低于对照组。与对照组相比，加入凝血酶后，第一组血小板在10秒时PIP2放射性显著降低。这些结果表明，患有视网膜病变的糖尿病患者血小板中多磷酸肌醇的分解增加，并且患有大血管病变的糖尿病患者血小板中多磷酸肌醇的形成减少。
