Katsuragi T, Kuratomi L, Sato C, Furukawa T
Department of Pharmacology, School of Medicine, Fukuoka University, Japan.
Eur J Pharmacol. 1991 Jul 9;199(3):303-7. doi: 10.1016/0014-2999(91)90493-a.
We evaluated the contractile reactivity to various stimuli, and the content and release of noradrenaline (NA) from a non-vascular tissue, the vas deferens, isolated from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). The concentration-contraction curves for NA in tissue from animals of two ages (10-25 weeks and 30-45 weeks) were shifted to the left in SHR as compared with in age-matched WKY, with significant differences at 1.0 and/or 10 microM of NA. Similarly, the amplitude of contraction produced by electrical stimulation at 4, 8 and 16 Hz in the tissue was much larger in SHR than in WKY. However, ATP (10-100 microM) evoked contractions of the tissue to a similar extent in both SHR and WKY. The electrically evoked contractions of vas deferens from both strains were inhibited by isoprenaline in an approximate dose-dependent and equipotent manner. The tissue NA content, determined by HPLC-ECD, was nearly same in both SHR and WKY. In addition, the same amount of NA was released from the vas deferens of both strains by electrical stimulation in the presence of 4-aminopyridine. The present findings indicate that the contractile response of vas deferens to stimulation of alpha 1-adrenoceptors, but not of beta-adrenoceptors or P2X-purinoceptors, is more pronounced in SHR than in WKY and that a response indicative of hypertension may also occur in non-vascular tissue as it does in vascular tissue.
我们评估了从自发性高血压大鼠（SHR）和Wistar-Kyoto大鼠（WKY）分离出的非血管组织输精管对各种刺激的收缩反应性，以及去甲肾上腺素（NA）的含量和释放情况。与年龄匹配的WKY相比，两个年龄组（10 - 25周和30 - 45周）动物组织中NA的浓度 - 收缩曲线在SHR中向左移动，在1.0和/或10 microM的NA浓度下存在显著差异。同样，在该组织中，4、8和16 Hz电刺激产生的收缩幅度在SHR中比在WKY中要大得多。然而，ATP（10 - 100 microM）在SHR和WKY中引起组织收缩的程度相似。两种品系输精管的电诱发收缩均被异丙肾上腺素以近似剂量依赖性且等效的方式抑制。通过高效液相色谱 - 电化学检测法测定的组织NA含量在SHR和WKY中几乎相同。此外，在存在4 - 氨基吡啶的情况下，通过电刺激，两种品系的输精管释放的NA量相同。目前的研究结果表明，输精管对α1 - 肾上腺素能受体刺激的收缩反应在SHR中比在WKY中更明显，而对β - 肾上腺素能受体或P2X嘌呤受体刺激的收缩反应则不然，并且表明高血压相关的反应也可能在非血管组织中发生，就像在血管组织中一样。
