Hudecová S, Krizanová O
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Gen Physiol Biophys. 1991 Jun;10(3):299-307.
The existence of dihydropyridine receptor in crayfish striated muscle was proved by Northern blot analysis and 3H PN 200--110 binding. The alpha 1 subunit is encoded by a 8300 nt mRNA population and is expressed as 190 kD protein in crayfish T-tubular system, which binds 3H PN 200--110 (Bmax 1.5 +/- 0.4 pmol/mg protein and KD 6.2 +/- 0.8 nmol/l). The purified protein is phosphorylated by cAMP-dependent protein kinase. The dihydropyridine receptor in crayfish striated muscle also contains alpha 2 subunit, which on Northern blot gives the same signal as the alpha 2 subunit from rabbit skeletal muscle.
通过Northern印迹分析和3H PN 200-110结合，证实了小龙虾横纹肌中存在二氢吡啶受体。α1亚基由一个8300 nt的mRNA群体编码，并在小龙虾T小管系统中表达为190 kD的蛋白质，该蛋白质与3H PN 200-110结合（Bmax为1.5±0.4 pmol/mg蛋白质，KD为6.2±0.8 nmol/l）。纯化的蛋白质可被cAMP依赖性蛋白激酶磷酸化。小龙虾横纹肌中的二氢吡啶受体还含有α2亚基，在Northern印迹上，其信号与兔骨骼肌的α2亚基相同。
