细菌转座子Tn7编码的转座蛋白的鉴定

Identification of transposition proteins encoded by the bacterial transposon Tn7.

作者信息

Orle K A, Craig N L

机构信息

Department of Microbiology and Immunology, University of California, San Francisco 94143.

出版信息

Gene. 1991 Jul 31;104(1):125-31. doi: 10.1016/0378-1119(91)90478-t.

DOI:10.1016/0378-1119(91)90478-t

PMID:1655576

Abstract

The bacterial transposon, Tn7, encodes an elaborate array of transposition genes, tnsABCDE. We report here the direct identification of the TnsA, TnsB, TnsC and TnsD polypeptides by immunoblotting. Our results demonstrate that the complexity of the protein information devoted to Tn7 transposition is considerable: the aggregate molecular size of the five Tns polypeptides is about 300 kDa. We also report the sequence of the tnsA gene and of the 5' ends of tnsB and tnsD. This analysis reveals that all five tns genes are oriented in the same direction within Tn7.

摘要

细菌转座子Tn7编码一系列复杂的转座基因，即tnsABCDE。我们在此报告通过免疫印迹法直接鉴定出TnsA、TnsB、TnsC和TnsD多肽。我们的结果表明，用于Tn7转座的蛋白质信息相当复杂：这五种Tns多肽的总分子大小约为300 kDa。我们还报告了tnsA基因以及tnsB和tnsD 5'端的序列。该分析表明，所有五个tns基因在Tn7内的方向相同。