van Wamel Annemieke, Kooiman Klazina, Harteveld Miranda, Emmer Marcia, ten Cate Folkert J, Versluis Michel, de Jong Nico
Department of Experimental Echocardiography, Thoraxcentre, Erasmus MC, Rotterdam, The Netherlands.
J Control Release. 2006 May 15;112(2):149-55. doi: 10.1016/j.jconrel.2006.02.007. Epub 2006 Mar 6.
Ultrasound contrast microbubbles have the ability to enhance endothelial cell permeability and thus may be used as a new way to deliver drugs. It facilitates the transfer of extracellular molecules into cells activated through ultrasound driven microbubbles. The present study is designed to correlate the relationship between microbubble induced cell deformation and enhanced cell membrane permeability. Propidium iodide (PI) was used as a membrane integrity probe. Using high-speed imaging of vibrating microbubbles against endothelial cells and imaging transport of PI into these cells showed a direct correlation between cell deformation and resulting cell membrane permeability. The membrane permeabilization lasted for a short period without affecting endothelial cells viability. We identified that microbubbles are crucial to enhance transient cell membrane permeability. Thus, permeability of individual cells is increased. The roles of ultrasound contrast microbubbles as the trigger for improved drug efficacy are discussed.
超声造影微泡具有增强内皮细胞通透性的能力，因此可作为一种新的药物递送方式。它有助于细胞外分子转移到通过超声驱动微泡激活的细胞中。本研究旨在关联微泡诱导的细胞变形与增强的细胞膜通透性之间的关系。碘化丙啶（PI）用作膜完整性探针。通过对振动微泡与内皮细胞进行高速成像以及对PI进入这些细胞的运输进行成像，结果表明细胞变形与由此产生的细胞膜通透性之间存在直接关联。膜通透性增加持续时间较短，且不影响内皮细胞的活力。我们确定微泡对于增强瞬时细胞膜通透性至关重要。因此，单个细胞的通透性增加。文中还讨论了超声造影微泡作为提高药物疗效触发因素的作用。
