Momiki S, Baba M, Caamano J, Iizasa T, Nakajima M, Yamaguchi Y, Klein-Szanto A
Department of Pathology, Fox Chase Cancer Center, Philadelphia, Pa.
Invasion Metastasis. 1991;11(2):66-75.
Eight cell lines derived from human non-small cell lung carcinomas were used to compare their in vivo invasiveness, in vitro chemoinvasive abilities and type IV collagenase activity. For the evaluation of the in vivo invasive potential, the tumor cells were seeded into deepithelialized rat tracheas and transplanted subcutaneously into nude mice. The invasive behavior of the cells was observed at 4, 8 and 12 weeks and assessed histologically by determination of the levels of penetration of tumor cells into the different layers of the tracheal wall. Except for two cell lines that did not grow at all in vivo, there was a very good correspondence between the levels of in vivo tracheal wall penetration and the in vitro chemoinvasion assay using fibronectin as chemoattractant and Matrigel as barrier. This also correlated very well with the capacity of the cells to secrete type IV collagenase. The in vivo evaluation of invasion using tracheal transplants, although requiring several weeks of experimentation, proved to be very reliable, yielding homogeneous results with little internal variation, and is proposed as a dependable in vivo invasion assay that closely mimics the in vivo human conditions in which most carcinomas develop and eventually invade neighboring tissues.
使用源自人非小细胞肺癌的8种细胞系来比较它们的体内侵袭性、体外化学侵袭能力和IV型胶原酶活性。为了评估体内侵袭潜能，将肿瘤细胞接种到去上皮的大鼠气管中，并皮下移植到裸鼠体内。在4周、8周和12周观察细胞的侵袭行为，并通过测定肿瘤细胞侵入气管壁不同层的程度进行组织学评估。除了两种细胞系在体内完全不生长外，体内气管壁穿透水平与使用纤连蛋白作为化学引诱剂和基质胶作为屏障的体外化学侵袭试验结果之间存在很好的对应关系。这也与细胞分泌IV型胶原酶的能力密切相关。使用气管移植进行侵袭的体内评估虽然需要数周的实验，但被证明非常可靠，产生的结果均匀，内部差异很小，并且被提议作为一种可靠的体内侵袭试验，它紧密模拟了大多数癌症发生并最终侵袭邻近组织的体内人类情况。
