Polette M, Clavel C, Muller D, Abecassis J, Binninger I, Birembaut P
INSERM U-314, Laboratoire Pol-Bouin, Hôpital Maison-Blanche, Reims, France.
Invasion Metastasis. 1991;11(2):76-83.
The mRNAs encoding 2 metalloproteinases, stromelysin 2 and collagenase I, have been detected by in situ hybridization in 26 carcinomas of the head and neck. 23 tumors of 26 expressed these mRNAs. Collagenase mRNAs were present in individual invasive cancer cells and in tumor cells at the periphery of poorly differentiated clusters (4 cases). Numerous stromal cells, principally fibroblasts were labeled (18 cases). Stromelysin mRNAs have been localized in tumor cells frequently arranged along disrupted basement membranes (8 cases). Many stromal cells in close contact to cancer cells also expressed the stromelysin mRNAs (17 cases). Normal residual cells were never labeled. These observations plead for the role of stromelysin produced by both stromal and tumor cells in the breakdown of basement membranes and the involvement of both collagenase and stromelysin in stromal invasion in carcinomas of the head and neck in vivo.
通过原位杂交技术，在26例头颈部癌中检测到了编码两种金属蛋白酶（基质溶解素2和胶原酶I）的信使核糖核酸（mRNA）。26例肿瘤中有23例表达了这些mRNA。胶原酶mRNA存在于单个浸润性癌细胞以及低分化癌巢周边的肿瘤细胞中（4例）。大量基质细胞，主要是成纤维细胞被标记（18例）。基质溶解素mRNA定位于常沿破坏的基底膜排列的肿瘤细胞中（8例）。与癌细胞紧密接触的许多基质细胞也表达基质溶解素mRNA（17例）。正常残余细胞从未被标记。这些观察结果表明，基质细胞和肿瘤细胞产生的基质溶解素在基底膜破坏中起作用，并且胶原酶和基质溶解素都参与了头颈部癌在体内的基质浸润。
