Polette M, Clavel C, Birembaut P, De Clerck Y A
I.N.S.E.R.M. U-314, Université de Reims, France.
Pathol Res Pract. 1993 Nov;189(9):1052-7. doi: 10.1016/S0344-0338(11)80679-5.
The presence and distribution of mRNAs encoding a matrix metalloproteinase (MMP) stromelysin 3 and two tissue inhibitors of MMP, TIMP1 and TIMP-2 have been studied by in situ hybridization of 18 human epidermoid head and neck carcinomas and four normal tissues. We found that in 16 tumors out of 18, stromelysin 3 mRNAs were only expressed by fibroblasts which were in close contact to invasive cancer cells. Tumor cells and normal tissues were not labeled. TIMP-1 mRNAs were detected in well differentiated cancer cells and in endothelial cells in all the cancers. In 13 out of the 18 carcinomas, TIMP-2 mRNAs were localized in only a few stromal cells near well differentiated invasive cancer cells and in endothelial cells. The significant expression of TIMP-1 and TIMP-2 mRNAS may lead to less aggressive MMPs, especially in the case of stromelysin 3, in the invasive process of the stroma.
通过对18例人表皮样头颈部癌和4例正常组织进行原位杂交，研究了编码基质金属蛋白酶（MMP）基质溶解素3以及两种MMP组织抑制剂TIMP1和TIMP - 2的mRNA的存在情况和分布。我们发现，在18例肿瘤中的16例中，基质溶解素3 mRNA仅由与浸润性癌细胞紧密接触的成纤维细胞表达。肿瘤细胞和正常组织未被标记。在所有癌症的高分化癌细胞和内皮细胞中均检测到TIMP - 1 mRNA。在18例癌中的13例中，TIMP - 2 mRNA仅定位在高分化浸润性癌细胞附近的少数基质细胞和内皮细胞中。TIMP - 1和TIMP - 2 mRNA的显著表达可能导致在基质浸润过程中MMP的侵袭性降低，尤其是在基质溶解素3的情况下。
