Asanuma N, Nomura H
Department of Oral Physiology, Matsumoto Dental College, Shiojiri, Japan.
Histochem J. 1991 Feb;23(2):83-90. doi: 10.1007/BF01047112.
Adenylate cyclase activity was demonstrated in the cilia, dendritic knob and axon of rat olfactory cells by using a strontium-based cytochemical method. The activity in the cilia and the dendritic knob was enhanced by non-hydrolyzable GTP (guanosine triphosphate) analogues and forskolin, and inhibited by Ca2+, all in agreement with biochemical reports of the odorant-sensitive adenylate cyclase. The results support the hypothesis of cyclic AMP working as a second messenger in olfactory transduction and imply that the transduction sites exist not only in the olfactory cilia but also in the dendritic knob. Enzymatic activity was also observed in the olfactory dendritic shaft by treating the tissue with 0.0002% Triton X-100, although the properties and role of the enzyme in this region are uncertain. The detergent inhibited the enzymatic activity in the cilia and the dendritic knob.
采用基于锶的细胞化学方法，在大鼠嗅觉细胞的纤毛、树突状小体和轴突中证实了腺苷酸环化酶活性。不可水解的鸟苷三磷酸（GTP）类似物和福斯可林可增强纤毛和树突状小体中的活性，而Ca2+可抑制该活性，所有这些均与嗅觉敏感腺苷酸环化酶的生化报告一致。这些结果支持环磷酸腺苷（cAMP）作为嗅觉转导中的第二信使的假说，并暗示转导位点不仅存在于嗅觉纤毛中，也存在于树突状小体中。通过用0.0002%的曲拉通X-100处理组织，在嗅觉树突干中也观察到了酶活性，尽管该区域中酶的特性和作用尚不确定。去污剂抑制了纤毛和树突状小体中的酶活性。
