Mami-Chouaib F, Del Porto P, Delorme D, Hercend T
Laboratoire d'Hémato-Immunologie, INSERM, U333, Institut Gustave Roussy, Villejuif, France.
J Immunol. 1991 Nov 1;147(9):2864-7.
We have demonstrated recently that a molecule, termed TCT.1 (Blast-1/CD48), is recognized on the surface of target cells by a series of alloreactive gamma/delta T cell clones generated from PBL of one healthy individual (designated E). Southern blot analyses suggested that these clones express a TCR associating a V3-JP2-C2 gamma-chain and V1-D-J1-C delta-chain. In the present study, we have developed from PBL of a second normal donor (designated G) a novel series of gamma/delta cloned T cell lines with similar functional activity (i.e., specific recognition of TCT.1 protein). The TCR gamma- and delta-chain nucleotide sequences of both the E and G clones were determined. Results show that 1) sequences from all the clones are identical in each individual donor, 2) the delta-chains expressed by the E and the G clones are encoded by distinct gene rearrangements including V1-D-J delta 1 and V1-D-J delta 2, respectively, 3) the gamma-chains expressed by the E and the G clones are encoded by the same genomic variable elements, namely V gamma 3 and JP2, whereas the junctional regions are distinct. Because the latter rearrangement is very infrequent in human peripheral blood, these data support the view that TCT.1/CD48 recognition is likely to be TCR dependent.
我们最近证明，一种名为TCT.1（Blast-1/CD48）的分子可被从一名健康个体（命名为E）的外周血淋巴细胞（PBL）中产生的一系列同种异体反应性γ/δT细胞克隆识别，这些克隆存在于靶细胞表面。Southern印迹分析表明，这些克隆表达一种与V3-JP2-C2γ链和V1-D-J1-Cδ链相关联的T细胞受体（TCR）。在本研究中，我们从第二名正常供体（命名为G）的PBL中培养出了一系列具有相似功能活性（即对TCT.1蛋白的特异性识别）的新型γ/δ克隆T细胞系。测定了E和G克隆的TCRγ链和δ链核苷酸序列。结果显示：1）每个个体供体的所有克隆序列均相同；2）E克隆和G克隆表达的δ链分别由不同的基因重排编码，包括V1-D-Jδ1和V1-D-Jδ2；3）E克隆和G克隆表达的γ链由相同的基因组可变元件编码，即Vγ3和JP2，而连接区不同。由于后一种重排在人外周血中非常罕见？，这些数据支持TCT.1/CD48识别可能依赖TCR的观点。 （注：原文中“Because the latter rearrangement is very infrequent in human peripheral blood”最后一个单词“blood”后标点符号有误，这里按“？”处理，翻译时保留原文错误情况）
