冷冻保存对动物实验模型中同种异体移植物直径和顺应性变化的影响。
The influence of cryopreservation on changes in diameter and compliance of allografts in an animal experimental model.
作者信息
Gabriel M, Wachal K, Dzieciuchowicz L, Pawlaczyk K, Krasiński Z, Oszkinis G
机构信息
Department of Vascular Surgery, University of Medical Science, Poznan, Poland.
出版信息
Eur J Vasc Endovasc Surg. 2006 Aug;32(2):169-75. doi: 10.1016/j.ejvs.2006.01.025. Epub 2006 Mar 29.
OBJECTIVE
The purpose of this study was to analyze the influence of cryopreservation on changes in diameter and compliance of allografts.
METHODS
Sixty aortic allografts implanted in situ in rats were analyzed. The animals were divided into four groups that received fresh or cryopreserved isogenic (Lewis to Lewis) grafts, or fresh or cryopreserved allogenic (Lewis to DA) grafts, respectively. The diameter and compliance of the grafts were then visually evaluated with the digital video camera recorder after 15, 30, 60, 90 and 120 days.
RESULTS
Gradual increase in diameter and decrease in compliance in case of all allogenic and cryopreserved isogenic grafts were observed. The observed changes in cryopreserved grafts were smaller when compared with fresh grafts, however, the differences did not reach statistical significance.
CONCLUSION
Cryo preservation does not protect allografts from stiffening and dilatation.
目的
本研究旨在分析冷冻保存对同种异体移植物直径和顺应性变化的影响。
方法
对原位植入大鼠体内的60个主动脉同种异体移植物进行分析。将动物分为四组，分别接受新鲜或冷冻保存的同基因（Lewis到Lewis）移植物，或新鲜或冷冻保存的异基因（Lewis到DA）移植物。然后在15、30、60、90和120天后，使用数字摄像机记录器对移植物的直径和顺应性进行视觉评估。
结果
观察到所有异基因和冷冻保存的同基因移植物的直径逐渐增加，顺应性降低。与新鲜移植物相比，冷冻保存移植物中观察到的变化较小，然而，差异未达到统计学意义。
结论
冷冻保存不能保护同种异体移植物免于硬化和扩张。
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