Dowdy S F, Fasching C L, Araujo D, Lai K M, Livanos E, Weissman B E, Stanbridge E J
Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.
Science. 1991 Oct 11;254(5029):293-5. doi: 10.1126/science.254.5029.293.
Wilms tumor has been associated with genomic alterations at both the 11p13 and 11p15 regions. To differentiate between the involvement of these two loci, a chromosome 11 was constructed that had one or the other region deleted, and this chromosome was introduced into the tumorigenic Wilms tumor cell line G401. When assayed for tumor-forming activity in nude mice, the 11p13-deleted, but not the 11p15.5-p14.1-deleted chromosome, retained its ability to suppress tumor formation. These results provide in vivo functional evidence for the existence of a second genetic locus (WT2) involved in suppressing the tumorigenic phenotype of Wilms tumor.
肾母细胞瘤与11p13和11p15区域的基因组改变有关。为了区分这两个位点的受累情况，构建了一条11号染色体，其中一个或另一个区域被删除，并将这条染色体导入致瘤性肾母细胞瘤细胞系G401。当在裸鼠中检测其肿瘤形成活性时，11p13缺失的染色体（而非11p15.5-p14.1缺失的染色体）保留了其抑制肿瘤形成的能力。这些结果为存在第二个参与抑制肾母细胞瘤致瘤表型的基因位点（WT2）提供了体内功能证据。
