Gazit Roi, Gruda Raizy, Elboim Moran, Arnon Tal I, Katz Gil, Achdout Hagit, Hanna Jacob, Qimron Udi, Landau Guy, Greenbaum Evgenia, Zakay-Rones Zichria, Porgador Angel, Mandelboim Ofer
The Lautenberg Center for General and Tumor Immunology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel.
Nat Immunol. 2006 May;7(5):517-23. doi: 10.1038/ni1322. Epub 2006 Mar 26.
The elimination of viruses and tumors by natural killer cells is mediated by specific natural killer cell receptors. To study the in vivo function of a principal activating natural killer cell receptor, NCR1 (NKp46 in humans), we replaced the gene encoding this receptor (Ncr1) with a green fluorescent protein reporter cassette. There was enhanced spread of certain tumors in 129/Sv but not C57BL/6 Ncr1(gfp/gfp) mice, and influenza virus infection was lethal in both 129/Sv and C57BL/6 Ncr1(gfp/gfp) mice. We noted accumulation of natural killer cells at the site of influenza infection by tracking the green fluorescent protein. Our results demonstrate a critical function for Ncr1 in the in vivo eradication of influenza virus.
自然杀伤细胞对病毒和肿瘤的清除是由特定的自然杀伤细胞受体介导的。为了研究主要的激活型自然杀伤细胞受体NCR1（人类中的NKp46）的体内功能，我们用绿色荧光蛋白报告基因盒替换了编码该受体的基因（Ncr1）。在129/Sv小鼠而非C57BL/6 Ncr1（gfp/gfp）小鼠中，某些肿瘤的扩散有所增强，并且流感病毒感染对129/Sv和C57BL/6 Ncr1（gfp/gfp）小鼠均具有致死性。通过追踪绿色荧光蛋白，我们注意到流感感染部位有自然杀伤细胞的聚集。我们的结果证明了Ncr1在体内清除流感病毒方面的关键作用。
