Takagi S, Takada K, Sairenji T
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
Virology. 1991 Nov;185(1):309-15. doi: 10.1016/0042-6822(91)90778-a.
The localizations of the Epstein-Barr virus immediate-early transcriptional activator BZLF1 protein ZEBRA, of the BMRF1 early antigen diffuse component (EA-D), and of viral DNA replication were studied in the Burkitt's lymphoma cell line Akata treated with anti-human immunoglobulin antibodies. Prompt and sequential appearance of ZEBRA, EA-D, and viral DNA was observed in about 70% of the cells. At early times after activation, ZEBRA had a diffuse intranuclear distribution, but later it was concentrated in globular regions within the nucleus. EA-D appeared first in a finely stippled pattern and then in a diffuse pattern. At late times, EA-D concentrated in globular regions similar to those with ZEBRA. Double staining for ZEBRA and EA-D revealed that ZEBRA followed the morphological changes of EA-D with a 1-2 hr delay and that both finally coalesced in the same structures, where in situ hybridization localized replicating viral DNA. The redistribution of both ZEBRA and EA-D to these compartments depended upon the replication of lytic viral DNA. These findings indicate that these globular regions are sites for viral replication and that transcription of EBV late genes may be regulated in these structures.
在使用抗人免疫球蛋白抗体处理的伯基特淋巴瘤细胞系Akata中，研究了爱泼斯坦-巴尔病毒即刻早期转录激活因子BZLF1蛋白ZEBRA、BMRF1早期抗原弥散成分（EA-D）的定位以及病毒DNA复制情况。在约70%的细胞中观察到ZEBRA、EA-D和病毒DNA迅速且依次出现。激活后的早期，ZEBRA呈弥散的核内分布，但随后集中于核内的球状区域。EA-D最初呈细点状分布，随后呈弥散分布。后期，EA-D集中于与ZEBRA相似的球状区域。对ZEBRA和EA-D进行双重染色显示，ZEBRA在形态变化上比EA-D延迟1 - 2小时，且二者最终在相同结构中合并，原位杂交显示这些结构中有正在复制的病毒DNA。ZEBRA和EA-D向这些区室的重新分布依赖于裂解性病毒DNA的复制。这些发现表明这些球状区域是病毒复制的位点，且EBV晚期基因的转录可能在这些结构中受到调控。
