Schubach W H, Horvath G, Spoth B, Hearing J C
Department of Medicine, State University of New York, Stony Brook 11790-8174.
Virology. 1991 Nov;185(1):428-31. doi: 10.1016/0042-6822(91)90792-a.
The open reading frame encoding the Epstein-Barr virus nuclear antigen 2 (EBNA-2) has been expressed in a recombinant baculovirus vector. The resulting product migrates with the same apparent molecular weight as EBNA-2 from latently infected or converted B cell lines. Rabbit antisera derived from the innoculation of this material immunoprecipitated EBNA-2 from cell extracts of EBV-containing cells. The high level of protein expression obtained in insect cells stands in sharp contrast to that seen in a number of mammalian cell lines using a variety of promoters including the endogenous EBNA-2 promoter, the Moloney MuLV LTR, the murine immunoglobulin heavy chain promoter, the human cytomegalovirus immediate early promoter, and the adenovirus major late promoter.
编码爱泼斯坦-巴尔病毒核抗原2（EBNA-2）的开放阅读框已在重组杆状病毒载体中表达。所得产物的迁移表观分子量与来自潜伏感染或转化的B细胞系的EBNA-2相同。用这种材料免疫接种产生的兔抗血清可从含EBV细胞的细胞提取物中免疫沉淀EBNA-2。在昆虫细胞中获得的高水平蛋白质表达与在多种哺乳动物细胞系中使用多种启动子（包括内源性EBNA-2启动子、莫洛尼鼠白血病病毒长末端重复序列、鼠免疫球蛋白重链启动子、人巨细胞病毒立即早期启动子和腺病毒主要晚期启动子）所观察到的情况形成鲜明对比。
