Chan Rodney K, Liu Perry H, Pietramaggiori Giorgio, Ibrahim Shahrul I, Hechtman Herbert B, Orgill Dennis P
Department of Surgery and the Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
J Burn Care Res. 2006 Mar-Apr;27(2):202-5. doi: 10.1097/01.BCR.0000202898.11277.58.
Burns, especially those involving large surface areas, represent a complex wound healing problem. Platelet-derived growth factor (PDGF) is released by activated platelets to recruit inflammatory cells toward the wound bed. It has effects on promoting angiogenesis and granulation tissue formation. However, the effectiveness of topical PDGF on wound closure is variable, ranging from little improvement observed in pig models to dramatic improvement reported in a diabetic mouse model. Here, we sought to determine the effectiveness of commercially sold PDGF-BB (Regranex) on wound closure in genetically diabetic mice. C57BL/KsJ db+/db+ mice and its host strain bearing dorsal 1.5-cm wounds were divided into groups (n = 8 in each group) receiving topical application of either Regranex (10 microg/wound) or vehicle for 5 consecutive days after wounding. The rate of wound closure was analyzed using computerized planimetry. The amount of granulation tissue was determined histologically. Our data indicate that diabetic mice exhibit a significant delay in wound closure when compared with their host strain. Topical application of Regranex did not improve the time to wound closure but did significantly increase the amount of granulation tissue. Our current study using commercially available Regranex failed to reproduce the previously reported finding that PDGF improved wound closure in healing impaired genetically diabetic mice.
烧伤，尤其是那些涉及大面积的烧伤，是一个复杂的伤口愈合问题。血小板衍生生长因子（PDGF）由活化的血小板释放，以吸引炎症细胞至伤口床。它具有促进血管生成和肉芽组织形成的作用。然而，局部应用PDGF促进伤口闭合的效果存在差异，从在猪模型中观察到的改善甚微到在糖尿病小鼠模型中报道的显著改善。在此，我们试图确定市售的PDGF-BB（Regranex）对遗传性糖尿病小鼠伤口闭合的有效性。将C57BL/KsJ db+/db+小鼠及其携带背部1.5厘米伤口的宿主品系分为几组（每组n = 8），在受伤后连续5天局部应用Regranex（10微克/伤口）或赋形剂。使用计算机化平面测量法分析伤口闭合率。通过组织学方法确定肉芽组织的量。我们的数据表明，与宿主品系相比，糖尿病小鼠伤口闭合明显延迟。局部应用Regranex并未缩短伤口闭合时间，但显著增加了肉芽组织的量。我们目前使用市售Regranex的研究未能重现先前报道的PDGF改善遗传性糖尿病小鼠愈合受损伤口闭合的发现。
