Duer Wayne C, Spitz Daniel J, McFarland Shallyn
Hillsborough County Medical Examiner Department, College of Medicine, University of South Florida, Tampa, FL 33602, USA.
J Forensic Sci. 2006 Mar;51(2):421-5. doi: 10.1111/j.1556-4029.2006.00063.x.
Cocaine is known to degrade in vivo and in vitro by several hydrolytic mechanisms. A previous study found that the initial amount of cocaine added to plasma could be accounted for by summing the molar concentrations of cocaine's hydrolysis products and the cocaine remaining after hydrolysis. The present study was undertaken to investigate whether or not relationships might exist between such molar concentration sums for different postmortem bodily fluids. Determinations of cocaine, benzoylecgonine, ecgonine methyl ester, and ecgonine were performed using liquid chromatography/mass spectrometry (LC/MS/MS) with heart blood, femoral blood, vitreous humor (VH), and urine (UR). The results demonstrate a strong correlation between blood and VH concentrations (correlation coefficients of 0.88-0.94), weak correlation between the UR and blood concentrations (correlation coefficients of 0.61-0.64), and weak correlation between UR and VH concentrations (correlation coefficient of 0.59). The results demonstrate that ecgonine is a significant hydrolysate with concentrations on the same order of magnitude as benzoylecgonine. The results are consistent with rapid distribution of the parent drug and its hydrolysates in the blood and VH. The strong correlation between the blood and VH demonstrates that VH is an important medium for toxicology testing when attempting to make a determination of cocaine intoxication.
众所周知，可卡因在体内和体外会通过多种水解机制降解。先前的一项研究发现，添加到血浆中的可卡因初始量可以通过将可卡因水解产物的摩尔浓度与水解后剩余的可卡因摩尔浓度相加来计算。本研究旨在调查不同死后体液的此类摩尔浓度总和之间是否可能存在关系。使用液相色谱/质谱联用仪（LC/MS/MS）对心血、股血、玻璃体液（VH）和尿液（UR）进行可卡因、苯甲酰芽子碱、芽子碱甲酯和芽子碱的测定。结果表明血液和VH浓度之间存在强相关性（相关系数为0.88 - 0.94），UR与血液浓度之间存在弱相关性（相关系数为0.61 - 0.64），UR与VH浓度之间存在弱相关性（相关系数为0.59）。结果表明芽子碱是一种重要的水解产物，其浓度与苯甲酰芽子碱处于同一数量级。结果与母体药物及其水解产物在血液和VH中的快速分布一致。血液和VH之间的强相关性表明，在试图确定可卡因中毒情况时，VH是毒理学检测的重要介质。
