Ebe Youji, Kuno Atsushi, Uchiyama Noboru, Koseki-Kuno Shiori, Yamada Masao, Sato Takashi, Narimatsu Hisashi, Hirabayashi Jun
Nano/Bio Science Research Lab., Yokohama Technical Center, Moritex Corporation, 1-3-3 Azamino-Minami, Aoba-ku, Yokohama, Kanagawa 225-0012.
J Biochem. 2006 Mar;139(3):323-7. doi: 10.1093/jb/mvj070.
We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.
我们最近基于倏逝场荧光检测原理开发了一种新型凝集素微阵列系统，通过该系统，即使是微弱的凝集素 - 寡糖相互作用也无需洗涤步骤即可检测到。为了其实际应用，对中国仓鼠卵巢（CHO）细胞进行了细胞聚糖分析，并将其聚糖谱与其糖基化缺陷的Lec突变体的聚糖谱进行了比较。每个细胞表面提取物都给出了与亲本CHO细胞显著不同的谱，其方式反映了所指出的生物合成特征。因此，所开发的凝集素微阵列系统被认为完全适用于粗样品的差异聚糖分析。
