淫羊藿黄酮对大鼠成骨细胞中骨保护素（OPG）及核因子κB受体活化因子配体（RANKL）表达的影响
[Effect of Herba Epimedii Flavone on expression of OPG and RANKL in rat osteoblasts].
作者信息
Liu Yiheng, Zang Hongmin, Zhang Haiying, Chen Junchang
机构信息
Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an.
出版信息
Zhong Yao Cai. 2005 Dec;28(12):1076-8.
OBJECTIVE
To explore the effect of Herba Epimedii Flavone (HEF) on expression of OPG and RANKL in rat osteoblasts.
METHODS
Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations (0.1, 1, 10, 100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR.
RESULTS
The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V(OPF)/V(RANKL) ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V(OPG)/V(RANKL) ratio was also decreased in the treatment group compared with that of the control group.
CONCLUSION
HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.
目的
探讨淫羊藿黄酮（HEF）对大鼠成骨细胞中骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）表达的影响。
方法
采用消化酶法从新生大鼠颅骨获取成骨细胞，在培养基中加入不同浓度的HEF（0.1、1、10、100 ng/ml）。48小时和96小时后从成骨细胞中提取总mRNA，采用逆转录聚合酶链反应（RT-PCR）检测OPG和RANKL的mRNA表达。
结果
与对照组相比，成骨细胞中OPG的mRNA表达显著增加，48小时后10 ng/ml处理组的V(OPF)/V(RANKL)比值明显降低。96小时后，与对照组相比，处理组中OPG的mRNA表达显著增加，V(OPG)/V(RANKL)比值也降低。
结论
HEF抑制成骨细胞的分化和成熟。HEF可预防和治疗骨质疏松症。
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