Liu Yi-Heng, Zhang Hai-Ying, Zang Hong-Min, Cheng Jun-Chang
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(6):487-90.
To explore the effect of Herba Epimedii flavone (HEF) on the osteoblast metabolism in vitro.
Osteoblast were obtained from new born rat calvaria by digestive enzymes. MTF, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.
It was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts (P < 0.01, P < 0.05).
HEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.
探讨淫羊藿黄酮（HEF）对体外成骨细胞代谢的影响。
采用消化酶法从新生大鼠颅骨获取成骨细胞。运用MTF、PNPP和RT-PCR法观察体外培养成骨细胞的增殖、碱性磷酸酶（ALP）活性以及骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）的mRNA表达。
发现HEF对体外培养成骨细胞具有促进细胞增殖、增强ALP活性以及上调OPG mRNA表达的作用（P<0.01，P<0.05）。
HEF可促进体外培养成骨细胞的增殖、分化以及OPG mRNA的表达。
