Wu Hongkai, Huang Bo, Zare Richard N
Department of Chemistry, Tsinghua University, Beijing, China.
J Am Chem Soc. 2006 Apr 5;128(13):4194-5. doi: 10.1021/ja058530o.
A microfluidic device is used to generate a complex gradient of diffusible molecules in a static solution. The gradient is precise and steady both in space and in time. This device, made from poly(dimethylsiloxane), consists of three layers. The molecules in reservoirs on the top layer diffuse through the flat middle layer of hydrogel and reach an equilibrium distribution. Microfluidic channels on the bottom layer that are in close contact with the hydrogel contain free solution that has concentration gradients based on the gradient in the gel. The gradient profile in the channel can be designed to have an arbitrary form (within the range of the existing gradient in the hydrogel) by controlling the local direction of the channel at each point.
一种微流控装置用于在静态溶液中生成可扩散分子的复杂梯度。该梯度在空间和时间上都是精确且稳定的。这种由聚二甲基硅氧烷制成的装置由三层组成。顶层储液器中的分子通过水凝胶的平坦中间层扩散并达到平衡分布。与水凝胶紧密接触的底层微流控通道包含基于凝胶中梯度的具有浓度梯度的自由溶液。通过控制通道在每个点的局部方向，通道中的梯度分布可以设计成具有任意形式（在水凝胶中现有梯度范围内）。
