抗菌肽多角体蛋白在处理后定位于大肠杆菌的细胞质中。
The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment.
作者信息
Powers Jon-Paul S, Martin Morgan M, Goosney Danika L, Hancock Robert E W
机构信息
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
出版信息
Antimicrob Agents Chemother. 2006 Apr;50(4):1522-4. doi: 10.1128/AAC.50.4.1522-1524.2006.
Abstract
The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.
摘要
鲎肽polyphemusin I具有很高的抗菌活性，但其作用机制尚未明确。我们使用生物素标记的polyphemusin I类似物和共聚焦荧光显微镜，发现该肽在添加后30分钟内积聚在野生型大肠杆菌的细胞质中，且不会造成实质性的膜损伤。
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