Tran Nguyen, Poussier Sylvain, Franken Philippe R, Maskali Fatiha, Groubatch Frederique, Vanhove Chris, Antunes Laurent, Karcher Gilles, Villemot Jean-Pierre, Marie Pierre-Yves
Laboratory of Surgery School, Faculty of Medicine, UHP-Nancy, Avenue de la forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France.
Eur J Nucl Med Mol Imaging. 2006 Jun;33(6):709-15. doi: 10.1007/s00259-006-0075-9. Epub 2006 Mar 30.
Cell therapy using bone marrow mesenchymal stem cells (BMSCs) shows promise in the treatment of myocardial infarction (MI) but accurate cell delivery within MI areas remains critical. In the present study, we tested the feasibility of in vivo pinhole SPECT imaging for monitoring the sites of intramyocardial implanted BMSCs in relation to targeted MI areas in rats.
BMSCs were labelled with (111)In-oxine and injected within the fibrotic areas of 3-month-old MI in ten rats. Two days later, dual (111)In/(99m)Tc-sestamibi pinhole SPECT was recorded for localisation of (111)In-BMSCs on a 15-segment left ventricular (LV) division. Additional (99m)Tc-sestamibi pinhole SPECT had been performed 1 month earlier and on the day before transplantation. In vitro counting on histological sections was used to validate the pinhole SPECT determination of (111)In-BMSC activity within LV segments.
The underperfused MI area (segments with <70% uptake) was stable between the (99m)Tc-sestamibi SPECT study recorded at 1 month (4.6+/-1.9 segments) and at 1 day (4.7+/-2.3 segments) before transplantation. (111)In-BMSCs were detected by dual-energy SPECT in 56 segments: 33 (59%) were underperfused MI segments but 23 (41%) were not (14 adjacent and nine remote segments). Finally, (111)In-labelled BMSCs were not detected in 14 out of the 47 (30%) underperfused MI segments.
When BMSCs are injected within MI areas in rats, sites of early cell retention do not always match the targeted MI areas. The dual-energy pinhole SPECT technique may be used for monitoring the sites of early retention of implanted BMSCs and the data obtained may have critical importance when analysing the effects of cardiac cell therapy.
使用骨髓间充质干细胞（BMSCs）进行细胞治疗在心肌梗死（MI）治疗中显示出前景，但在MI区域内准确的细胞递送仍然至关重要。在本研究中，我们测试了体内针孔SPECT成像监测大鼠心肌内植入的BMSCs位点与靶向MI区域关系的可行性。
用（111）In-奥曲肽标记BMSCs，并将其注射到10只3月龄MI大鼠的纤维化区域内。两天后，记录双能量（111）In/（99m）Tc-司他比 pinhole SPECT，以在15节段左心室（LV）分区上定位（111）In-BMSCs。1个月前和移植前一天进行了额外的（99m）Tc-司他比 pinhole SPECT。对组织学切片进行体外计数，以验证针孔SPECT对LV节段内（111）In-BMSC活性的测定。
灌注不足的MI区域（摄取<70%的节段）在移植前1个月（4.6±1.9节段）和1天（4.7±2.3节段）记录的（99m）Tc-司他比SPECT研究之间是稳定的。通过双能SPECT在56个节段中检测到（111）In-BMSCs：33个（59%）是灌注不足的MI节段，但23个（41%）不是（14个相邻节段和9个远离节段）。最后，在47个（30%）灌注不足的MI节段中的14个中未检测到（111）In标记的BMSCs。
当将BMSCs注射到大鼠的MI区域内时，早期细胞滞留位点并不总是与靶向MI区域匹配。双能针孔SPECT技术可用于监测植入的BMSCs早期滞留位点，并且在分析心脏细胞治疗效果时获得的数据可能具有至关重要的意义。
